PMID: 557991May 2, 1977Paper

Quantitative studies on lysolecithin-mediated hemolysis. Use of ether-deoxy lysolecithin analogs with varying aliphatic chain-lengths

Biochimica Et Biophysica Acta
H U WeltzienR Reuther

Abstract

The process of lysolecithin-mediated hemolysis has been investigated by use of various ether-deoxy lysolecithin analogs (1-alkyl-propanediol-3-phosphorylcholine) with alkyl residues of 10-22 carbon atoms. Hemolytic activities were defined either as molar amounts to be added for 50% lysis (L50) or as cell-bound amounts at 50% lysis (A50). It was found, that in contrast to L50, A50 values are independent of experimental conditions. Moreover, L50 values primarily reflect the binding affinities, while A50 values give more accurate information on the actual membrane-disturbing potential. The strongest hemolytic C16-lysolecithin analog required 2 - 10(7) or 5 - 10(7) molecules bound per cell for 50% lysis at 0 or 37degrees C, respectively, corresponding to about 10 or 25% of the total membrane phospholipids. Evidence is presented, indicating that (a) lysophosphatides bind to cells below their critical micelle concentration, (b) micelles themselves are not generally necessary for cell lysis. Red cells of different species (man and cattle) as well as at varying temperatures exhibit significantly different sensitivities in terms of L50 and A50 values. These differences, however, depend on the degree of hydrophobicity of the lysolecithin...Continue Reading

References

Jun 7, 1973·Biochimica Et Biophysica Acta·H U Weltzien
Mar 1, 1952·The Journal of General Physiology·H B COLLIER

❮ Previous
Next ❯

Citations

Jan 1, 1983·The International Journal of Biochemistry·M Serres-Guillaumond, P Louisot
Apr 17, 1999·International Journal of Pharmaceutics·G StensrudJ Karlsen
Aug 1, 1992·The Journal of Pharmacy and Pharmacology·G P MartinC Marriott
Oct 12, 2013·Journal of Lipid Research·Bhaswati SenguptaSampath Parthasarathy
Jan 1, 1991·Journal of Biochemical Toxicology·W T ShierH H Wang
Jul 11, 2006·Magnetic Resonance in Chemistry : MRC·Charles R Sanders, Frank Sönnichsen
May 1, 1995·Metabolism: Clinical and Experimental·B CantinP Julien
Jun 22, 1988·Biochimica Et Biophysica Acta·G A SenisterraJ J Gagliardino
Mar 1, 1988·European Journal of Biochemistry·G Swoboda, W Hasselbach
Jul 9, 2002·Biochimica Et Biophysica Acta·Jesper DavidsenKent Jørgensen
Jul 19, 1979·Biochimica Et Biophysica Acta·T J BierbaumW H Huestis
Nov 25, 2000·The Journal of Pharmacy and Pharmacology·L A GouldG P Martin
Dec 8, 1995·The Journal of Biological Chemistry·S Günther-AusbornT Stegmann
Apr 17, 1999·International Journal of Pharmaceutics·G StensrudJ Karlsen
Aug 20, 1979·Biochimica Et Biophysica Acta·H U Weltzien
Sep 11, 1986·Biochimica Et Biophysica Acta·B IsomaaA C Engblom
Apr 27, 1979·Biochimica Et Biophysica Acta·S ParthasarathyW J Baumann
Jul 25, 1978·Biochimica Et Biophysica Acta·B ArnoldH U Weltzien
Mar 28, 2003·Biochimica Et Biophysica Acta·Aravind Asokan, Moo J Cho

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.