PMID: 6965304Jan 1, 1980Paper

Quantitative studies on the precursors of cytotoxic lymphocytes. VI. Second signal requirements of specifically activated precursors isolated 12 h after stimulation

The Journal of Experimental Medicine
M E LalandeR G Miller

Abstract

It is shown that, in a mixed lymphocyte reaction, the production of cytotoxic T lymphocytes (CL) from cytotoxic T lymphocyte precursors (CLP) requires two signals which are separated time. Using a flow cytometer-cell sorter and a vital, fluorescent DNA stain, Hoechst 3342, CLP specific for the stimulator cells can be separated from other CLP and from stimulator cells 12 h after initiation of mixed lymphocyte cultures. These CLP are in a state of partial activation and can produce CL in the absence of stimulator cells if a second signal in the form of a concanavalin A-induced spleen cell supernate factor is added. Specific CL are also generated when the partially activated CLP are cultured with both nude spleen cells and stimulator cells. In this case it appears that an interaction between the stimulator cells and the nude spleen cells leads to production of the second signal.

References

Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D J Arndt-Jovin, T M Jovin
Jan 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M E Lalande, R G Miller
Jul 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G B PriceR G Miller
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D W TalmageK J Lafferty
Jan 1, 1977·Immunological Reviews·R G MillerR A Phillips
Jan 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S A Latt, G Stetten
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg

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Citations

Jul 31, 1998·Medical & Biological Engineering & Computing·R Tran-Son-TayW Shyy
Jan 1, 1981·Journal of Immunological Methods·R G MillerG B Price
Nov 16, 1984·Journal of Immunological Methods·M Brenan, C R Parish
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M E LalandeR G Miller
Mar 1, 1982·The Journal of Experimental Medicine·J A Keene, J Forman
Jan 1, 1981·Immunological Reviews·J KornbluthB Dupont
Apr 1, 1987·Scandinavian Journal of Immunology·G GromoF H Bach
Jan 1, 1980·Immunological Reviews·V PaetkauB Caplan

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