PMID: 6986901Feb 5, 1980Paper

Quantitative study of the fluidity of Escherichia coli membranes using deuterium magnetic resonance

Biochemistry
C P NicholM Bloom

Abstract

Specifically deuterated palmitic acid was incorporated into the membrane phospholipids of the L51 strain of Escherichia coli. The cytoplasmic and outer membranes were separated by using standard techniques and studied by deuterium nuclear magnetic resonance between 0 and 40 degrees C. Distinctive liquid-crystalline and gel spectra were observed to coexist over a wide temperature range. The relative intensities of these spectra provided a direct measure of the fraction of the deuterium-labeled phospholipids in the fluid state as a function of temperature. Above 37 degrees C, the amount of immobilized or gel-phase phospholipid is estimated to be less than 3% of the total phospholipid. The gel to liquid-crystalline transition region for the outer membrane was shifted upwards by approximately 7 degrees C relative to that of the cytoplasmic membrane, in agreement with previous studies [Davis, J. H., Nichol, C. P., Weeks, G., & Bloom, M. (1979) Biochemistry 18, 2103]. The orientational order in the fluid phase of both membranes decreased gradually with increasing temperature and was greater in the outer membrane than in the cytoplasmic membrane. The orientational order of the gel-phase component was the same for both membranes, withi...Continue Reading

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Citations

Aug 14, 1980·Biochemical and Biophysical Research Communications·A S JanoffE J McGroarty
Mar 1, 1991·Chemistry and Physics of Lipids·A Watts, P J Spooner
Jan 1, 1981·Progress in Biophysics and Molecular Biology·P J Quinn
Mar 21, 1983·Biochimica Et Biophysica Acta·J H Davis
Jul 9, 1999·Progress in Lipid Research·C C DiRussoJ D Weimar
Jan 1, 1983·Experimental Pathology·H W Meyer
Feb 1, 1980·Quarterly Reviews of Biophysics·J Seelig, A Seelig
Mar 25, 1983·Journal of Molecular Biology·E D Muller, J E Cronan
Mar 23, 1982·Biochimica Et Biophysica Acta·B MaravigliaK W Wirtz
Jun 14, 1982·Biochimica Et Biophysica Acta·H C JarrellI C Smith
Sep 20, 2012·Biochimica Et Biophysica Acta·Catherine Tardy-LaporteIsabelle Marcotte
May 6, 2003·Biochimica Et Biophysica Acta·A G Lee
Jun 6, 2020·The Journal of Biological Chemistry·Jim E HorneSheena E Radford
Apr 20, 2018·BMC Microbiology·Katherine M LupinoGregory B Hecht
Dec 13, 2019·Biochimica Et Biophysica Acta. Biomembranes·Jingjing GuoThomas Seviour
Jan 1, 1984·Journal of Bacteriology·K M Dombek, L O Ingram
Mar 1, 1985·Microbiological Reviews·H Nikaido, M Vaara
Jul 20, 1984·Science·R L Smith, E Oldfield
Nov 5, 2021·Nature Reviews. Microbiology·Jiawei SunKerwyn Casey Huang

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