Quantitative Succinyl-Proteome Profiling of Camellia sinensis cv. 'Anji Baicha' During Periodic Albinism

Scientific Reports
Yan-Xia XuLiang Chen

Abstract

Lysine succinylation is a novel dynamic and evolutionarily conserved post-translational modification (PTM) that regulates various biological processes. 'Anji Baicha' is an albino tea variety that exhibits temperature-based variability of leaf colour and amino acid concentrations. However, the mechanism underlying albinism in 'Anji Baicha' has not been investigated at the level of succinylation. Here, we identify 3530 lysine succinylation sites mapped to 2132 proteins in 'Anji Baicha', representing the first extensive data on the lysine succinylome in the tea plant. Eleven conserved succinylation motifs were enriched among the identified succinylated peptides. The protein-protein interaction maps were visualized using Cytoscape software. Comparison across three typical developmental stages of 'Anji Baicha' revealed that proteins exhibiting differential succinylation levels were primarily involved in photosynthesis, carbon fixation, biosynthesis of amino acids and porphyrin and chlorophyll metabolism, suggesting that these succinylated proteins are involved in 'Anji Baicha' leaf colour variability. These results not only deepen our understanding of the mechanism underlying 'Anji Baicha' albinism and the regulatory role of succiny...Continue Reading

References

Nov 29, 1965·Biochimica Et Biophysica Acta·J F Wintermans, A de Mots
Nov 24, 2004·FEBS Letters·Ran RosenEliora Z Ron
Nov 4, 2005·Angewandte Chemie·Christopher T WalshGregory J Gatto
May 15, 2007·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Tao ChenCailin Wang
Nov 17, 2009·Journal of the American Chemical Society·Michael G AckerChristopher T Walsh
Dec 15, 2010·Nature Chemical Biology·Zhihong ZhangYingming Zhao
Mar 6, 2012·Molecular & Cellular Proteomics : MCP·Zhongyu XieYingming Zhao
Aug 21, 2012·Trends in Endocrinology and Metabolism : TEM·Wenjuan HeEric Verdin
Jun 29, 2013·Molecular Cell·Jeongsoon ParkYingming Zhao
Nov 12, 2013·Nucleic Acids Research·Minoru KanehisaMao Tanabe
Dec 10, 2013·Cell Metabolism·Matthew J RardinEric Verdin
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Khizar HayatSobia Mushtaq
Oct 18, 2015·Nucleic Acids Research·Minoru KanehisaMao Tanabe
May 22, 2016·Journal of Experimental Botany·Chenjia ShenHuizhong Wang

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Citations

May 31, 2018·Scientific Reports·Kaidong LiuXiaolin Zheng
Dec 10, 2019·Horticulture Research·Xiaobing WangLikai Chen
Apr 20, 2021·Frontiers in Plant Science·Juncheng WangHuajun Wang
Feb 6, 2018·Journal of Agricultural and Food Chemistry·Chun-Fang LiLiang Chen
Apr 18, 2020·Journal of Agricultural and Food Chemistry·Haiji QiuWeiwei Wen

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Methods Mentioned

BETA
acetylation
PCR
electrophoresis
protein modification

Software Mentioned

Cytoscape
Genesis
DAVID Bioinformatics Resources
gplots
Andromeda
R
Motif
MaxQuant

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