Quantitative SUMO proteomics reveals the modulation of several PML nuclear body associated proteins and an anti-senescence function of UBC9

Scientific Reports
Francis P McManusPierre Thibault

Abstract

Several regulators of SUMOylation have been previously linked to senescence but most targets of this modification in senescent cells remain unidentified. Using a two-step purification of a modified SUMO3, we profiled the SUMO proteome of senescent cells in a site-specific manner. We identified 25 SUMO sites on 23 proteins that were significantly regulated during senescence. Of note, most of these proteins were PML nuclear body (PML-NB) associated, which correlates with the increased number and size of PML-NBs observed in senescent cells. Interestingly, the sole SUMO E2 enzyme, UBC9, was more SUMOylated during senescence on its Lys-49. Functional studies of a UBC9 mutant at Lys-49 showed a decreased association to PML-NBs and the loss of UBC9's ability to delay senescence. We thus propose both pro- and anti-senescence functions of protein SUMOylation.

References

May 31, 1990·Nature·C B HarleyC W Greider
Oct 3, 1998·The Journal of Biological Chemistry·T KamitaniE T Yeh
Mar 13, 1999·The Journal of Biological Chemistry·A C LeeT Finkel
Feb 29, 2000·The Journal of Biological Chemistry·H Saitoh, J Hinchey
Nov 18, 2000·The EMBO Journal·V FogalG Del Sal
Mar 30, 2002·Science·Jan KarlsederTitia de Lange
Jul 3, 2002·The EMBO Journal·Oliver BischofAnne Dejean
May 2, 2003·Nature Reviews. Cancer·Judith Campisi
Oct 20, 2004·Cell Cycle·Teeru BihaniAthena W Lin
Aug 19, 2005·The EMBO Journal·Chuangui WangJiandong Chen
Mar 10, 2006·The Journal of Biological Chemistry·Christina-Maria HeckerIvan Dikic
Jun 24, 2006·Molecular Cell·Oliver BischofAnne Dejean
Nov 4, 2006·Molecular Cell·Tian Huai ShenPier Paolo Pandolfi
Dec 13, 2006·The Journal of Cell Biology·Ryo FunayamaFuyuki Ishikawa
Mar 3, 2007·Molecular Biology of the Cell·Keiko TakahashiNorimitsu Inoue
May 12, 2007·The EMBO Journal·Puck KnipscheerTitia K Sixma
Aug 2, 2007·Nature Reviews. Molecular Cell Biology·Judith Campisi, Fabrizio d'Adda di Fagagna
Nov 15, 2007·Nature Reviews. Molecular Cell Biology·Ruth Geiss-Friedlander, Frauke Melchior
Apr 11, 2008·Nature·Erik Meulmeester, Frauke Melchior
Dec 25, 2008·Methods in Molecular Biology·Andreas WernerFrauke Melchior
Jan 21, 2010·International Journal of Biological Sciences·Ellen Van DammeXaveer Van Ostade
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Valérie Lallemand-Breitenbach, Hugues de Thé
Nov 26, 2010·Molecular & Cellular Proteomics : MCP·Frederic GalissonPierre Thibault
Jan 6, 2011·Genes & Development·Mathieu VernierGerardo Ferbeyre
Apr 14, 2011·FEBS Letters·Hong DouEdward T H Yeh
Jun 23, 2011·Science Signaling·Michael H TathamRonald T Hay
Dec 6, 2011·Cancer Letters·Kira BettermannJohannes Haybaeck
Dec 14, 2011·Genes & Development·Takeshi ShimiRobert D Goldman
Feb 22, 2012·Methods in Molecular Biology·Annette FlothoFrauke Melchior
May 1, 2012·Bioinformatics·Xiaoli JiaoRichard A Lempicki
Dec 4, 2012·Nucleic Acids Research·Andrea FranceschiniLars J Jensen
Apr 20, 2013·Genes & Development·Xavier Deschênes-SimardGerardo Ferbeyre
Aug 24, 2013·Cell Cycle·Laura Marcos-VillarCarmen Rivas
Oct 8, 2013·Nature Methods·Joseph P O'SheaDaniel Schwartz
Jan 23, 2014·Genes & Development·Rafik SalamaMasashi Narita
Mar 19, 2014·The Journal of Cell Biology·Umut SahinValérie Lallemand-Breitenbach

❮ Previous
Next ❯

Citations

Feb 23, 2019·PloS One·Fernando Josa-PradoKevin A Wilkinson
Feb 13, 2020·Nature Communications·Chongyang LiPierre Thibault
Aug 12, 2020·Fly·Sushmitha HegdeGirish S Ratnaparkhi
Jan 28, 2021·Critical Reviews in Analytical Chemistry·Negin Fasih RamandiHassan Y Aboul-Enein
Feb 5, 2021·Ageing Research Reviews·Rashmi Upadhyay PathakRakesh Kumar Mishra
Feb 11, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mathias BoulangerGuillaume Bossis
Apr 4, 2021·International Journal of Molecular Sciences·Xiaodi DuGuangyou Yang
May 11, 2021·Frontiers in Cell and Developmental Biology·Mounira K Chelbi-Alix, Pierre Thibault

❮ Previous
Next ❯

Datasets Mentioned

BETA
PASS01172

Methods Mentioned

BETA
PCR
immunoprecipitation
Acetylation
that
ubiquitination
Assay

Software Mentioned

MaxQuant
MCODE
PhosphoSite Plus
pLOGO
Perseus

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.