Quantitative target analysis and kinetic profiling of acyl-CoAs reveal the rate-limiting step in cyanobacterial 1-butanol production

Metabolomics : Official Journal of the Metabolomic Society
Shingo NoguchiEiichiro Fukusaki

Abstract

Cyanobacterial 1-butanol production is an important model system for direct conversion of CO2 to fuels and chemicals. Metabolically-engineered cyanobacteria introduced with a heterologous Coenzyme A (CoA)-dependent pathway modified from Clostridium species can convert atmospheric CO2 into 1-butanol. Efforts to optimize the 1-butanol pathway in Synechococcus elongatus PCC 7942 have focused on the improvement of the CoA-dependent pathway thus, probing the in vivo metabolic state of the CoA-dependent pathway is essential for identifying its limiting steps. In this study, we performed quantitative target analysis and kinetic profiling of acyl-CoAs in the CoA-dependent pathway by reversed phase ion-pair liquid chromatography-triple quadrupole mass spectrometry. Using (13)C-labelled cyanobacterial cell extract as internal standard, measurement of the intracellular concentration of acyl-CoAs revealed that the reductive reaction of butanoyl-CoA to butanal is a possible rate-limiting step. In addition, improvement of the butanoyl-CoA to butanal reaction resulted in an increased rate of acetyl-CoA synthesis by possibly compensating for the limitation of free CoA species. We inferred that the efficient recycling of free CoA played a key r...Continue Reading

References

Dec 30, 1968·Biochemical and Biophysical Research Communications·E VanderwinkelS J Ajl
Jan 30, 1999·Applied and Environmental Microbiology·M D Deng, J R Coleman
Jan 21, 2000·Journal of Applied Physiology·J S FisherM Brown
Apr 30, 2005·The Plant Journal : for Cell and Molecular Biology·Masahiro TamoiShigeru Shigeoka
Oct 10, 2007·Biotechnology Journal·Peter Dürre
Aug 21, 2008·Nature Protocols·Bryson D BennettJoshua D Rabinowitz
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
Jun 23, 2009·Biotechnology Advances·R GheshlaghiC P Chou
Nov 17, 2009·Nature Biotechnology·Shota AtsumiJames C Liao
Mar 15, 2011·Applied and Environmental Microbiology·Claire R ShenJames C Liao
Apr 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Xinyao LiuRoy Curtiss
May 17, 2011·Metabolic Engineering·Ethan I Lan, James C Liao
Mar 27, 2012·Journal of Biotechnology·Iara M P Machado, Shota Atsumi
Apr 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ethan I Lan, James C Liao
Nov 28, 2012·Applied and Environmental Microbiology·Arul M VarmanYinjie J Tang
Jan 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·John W K OliverShota Atsumi
May 7, 2013·Biotechnology for Biofuels·Weihua WangXuefeng Lu
May 10, 2013·Journal of Experimental Botany·Tomohisa HasunumaAkihiko Kondo
Feb 18, 2014·Biotechnology Advances·Hu JinWeiwen Zhang
Jul 2, 2014·Biotechnology and Bioengineering·András PásztorPatrik R Jones

❮ Previous
Next ❯

Citations

Apr 21, 2016·Biotechnology for Biofuels·Nadin PadeMartin Hagemann
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Sastia Prama PutriEiichiro Fukusaki
Sep 23, 2019·Journal of Bioscience and Bioengineering·Artnice Mega FathimaEiichiro Fukusaki
Sep 7, 2021·World Journal of Microbiology & Biotechnology·Pengfei GuQiang Li

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

BUOH
LabSolutions

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.