Quantitative temporal analysis of protein dynamics in cardiac remodeling

Journal of Molecular and Cellular Cardiology
Daniel B McClatchyJohn R Yates

Abstract

Cardiac remodeling (CR) is a complex dynamic process common to many heart diseases. CR is characterized as a temporal progression of global adaptive and maladaptive perturbations. The complex nature of this process clouds a comprehensive understanding of CR, but greater insight into the processes and mechanisms has potential to identify new therapeutic targets. To provide a deeper understanding of this important cardiac process, we applied a new proteomic technique, PALM (Pulse Azidohomoalanine in Mammals), to quantitate the newly-synthesized protein (NSP) changes during the progression of isoproterenol (ISO)-induced CR in the mouse left ventricle. This analysis revealed a complex combination of adaptive and maladaptive alterations at acute and prolonged time points including the identification of proteins not previously associated with CR. We also combined the PALM dataset with our published protein turnover rate dataset to identify putative biochemical mechanisms underlying CR. The novel integration of analyzing NSPs together with their protein turnover rates demonstrated that alterations in specific biological pathways (e.g., inflammation and oxidative stress) are produced by differential regulation of protein synthesis and ...Continue Reading

Citations

Apr 12, 2019·Current Opinion in Cardiology·Scot J Matkovich
May 30, 2019·Journal of Biological Engineering·Aya M SalehTamara L Kinzer-Ursem
Oct 1, 2020·Scientific Reports·Daniel B McClatchyJohn R Yates
Jun 9, 2020·Proteomics. Clinical Applications·Ricardo Cambraia ParreiraThiago Verano-Braga
Jan 25, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhanwu HouHuadong Liu
Feb 20, 2021·Biochimica Et Biophysica Acta. Proteins and Proteomics·Diana Paola Gómez-MendozaThiago Verano-Braga
Nov 5, 2020·Journal of Proteome Research·Yuanhui MaJohn R Yates
Aug 28, 2021·International Journal of Molecular Sciences·Luc RochetteCatherine Vergely

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