Quantitative top-down proteomics of SILAC labeled human embryonic stem cells

Journal of the American Society for Mass Spectrometry
Timothy S CollierDavid C Muddiman


Human embryonic stem cells (hESCs) are self-renewing pluripotent cells with relevance to treatment of numerous medical conditions. However, a global understanding of the role of the hESC proteome in maintaining pluripotency or triggering differentiation is still largely lacking. The emergence of top-down proteomics has facilitated the identification and characterization of intact protein forms that are not readily apparent in bottom-up studies. Combined with metabolic labeling techniques such as stable isotope labeling by amino acids in cell culture (SILAC), quantitative comparison of intact protein expression under differing experimental conditions is possible. Herein, quantitative top-down proteomics of hESCs is demonstrated using the SILAC method and nano-flow reverse phase chromatography directly coupled to a linear-ion-trap Fourier transform ion cyclotron resonance mass spectrometer (nLC-LTQ-FT-ICR-MS). In this study, which to the best of our knowledge represents the first top-down analysis of hESCs, we have confidently identified 11 proteins by accurate intact mass, MS/MS, and amino acid counting facilitated by SILAC labeling. Although quantification is challenging due to the incorporation of multiple labeled amino acids ...Continue Reading


Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·A ShevchenkoM Mann
Sep 15, 1998·Analytical Biochemistry·R L Winston, M C Fitzgerald
Jan 13, 2000·Journal of the American Society for Mass Spectrometry·T D VeenstraR D Smith
Oct 3, 2001·Nature Biotechnology·C XuM K Carpenter
Nov 28, 2001·Analytical Chemistry·R ZhangF E Regnier
Jan 24, 2002·Journal of the American Chemical Society·Ying GeFred W McLafferty
Jan 29, 2002·Journal of Mass Spectrometry : JMS·Suzana MartinovićRichard D Smith
Jun 12, 2004·Analytical Chemistry·Neil L Kelleher
Jul 16, 2004·Journal of Proteome Research·Samir Julka, Fred Regnier
Sep 25, 2004·Mass Spectrometry Reviews·Bogdan Bogdanov, Richard D Smith
Apr 20, 2005·Journal of Mass Spectrometry : JMS·Qizhi HuR Graham Cooks
May 25, 2005·Journal of Chromatography. a·Yueju WangCheng S Lee
Jan 18, 2006·Analytical Chemistry·John R YatesVlad Zabrouskov
Jun 26, 2007·Nucleic Acids Research·Leonid ZamdborgNeil L Kelleher
Sep 29, 2007·Nature Methods·Nertila Siuti, Neil L Kelleher
Oct 9, 2007·Journal of the American Society for Mass Spectrometry·Leonie F WaandersMatthias Mann
Nov 30, 2007·Molecular & Cellular Proteomics : MCP·Andrew J K WilliamsonAnthony D Whetton
Dec 28, 2007·Experimental & Molecular Medicine·Moon-Young ChoiSung Won Kwon
Mar 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Doug PhanstielJoshua J Coon
May 20, 2008·Molecular & Cellular Proteomics : MCP·Sean C BendallGilles A Lajoie
Jan 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Danielle L SwaneyJoshua J Coon
Jan 24, 2009·Journal of Muscle Research and Cell Motility·Raquel Sancho SolisJeffery W Walker
Mar 10, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Genna L AndrewsDavid C Muddiman
Jul 4, 2009·Analytical Chemistry·John C Tran, Alan A Doucette
Aug 12, 2009·Cell Stem Cell·Dennis Van HoofJeroen Krijgsveld
Sep 15, 2009·Journal of the American Society for Mass Spectrometry·Ji Eun LeeNeil L Kelleher

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