Quantitative Trait Locus Mapping of Clubroot Resistance and Plasmodiophora brassicae Pathotype Banglim-Specific Marker Development in Brassica rapa.

International Journal of Molecular Sciences
Su Ryun ChoiYong Pyo Lim

Abstract

Clubroot resistance is an economically important trait in Brassicaceae crops. Although many quantitative trait loci (QTLs) for clubroot resistance have been identified in Brassica, disease-related damage continues to occur owing to differences in host variety and constant pathogen variation. Here, we investigated the inheritance of clubroot resistance in a double haploid population developed by crossing clubroot resistant and susceptible lines "09CR500" and "09CR501", respectively. The resistance of "09CR500" to Plasmodiophora brassicae pathotype "Banglim" was controlled as a single dominant gene, with the segregation of resistance and susceptibility being nearly 1:1. PbBrA08Banglim was identified as having a logarithm of odds value of 7.9-74.8, and a phenotypic variance of 26.0-97.1% with flanking marker "09CR.11390652" in A08. After aligning QTL regions to the B. rapa reference genome, 11 genes were selected as candidates. PbBrA08Banglim was located near Crr1, CRs, and Rcr9 loci, but differences were validated by marker analysis, gene structural variations, and gene expression levels, as well as phenotypic responses to the pathotype. Genotyping using the "09CR.11390652" marker accurately distinguished the Banglim-resistance p...Continue Reading

References

Jan 1, 1996·Genetics·R W Doerge, G A Churchill
May 16, 2002·The Journal of Heredity·R E Voorrips
Sep 5, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·K SuwabeM Hirai
Oct 11, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M HiraiS Matsumoto
Mar 5, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Z Y PiaoY P Lim
Oct 14, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M SaitoM Hirai
Nov 28, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S WernerC Jung
Jul 10, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Koji SakamotoEtsuo Matsumoto
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Aug 15, 2009·Trends in Biotechnology·Rajeev K VarshneyScott A Jackson
Dec 17, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Akito KameiMasashi Hirai
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Oct 12, 2012·Plant Molecular Biology·Hiroki UenoNobuaki Hayashida
Apr 1, 2014·Current Opinion in Biotechnology·Marie E BolgerKlaus F X Mayer
Nov 15, 2015·Plant Science : an International Journal of Experimental Plant Biology·Rajeev K Varshney
Nov 23, 2017·Frontiers in Plant Science·Ting Xiang NeikJacqueline Batley
Aug 10, 2019·Molecular Breeding : New Strategies in Plant Improvement·Ondrej HejnaVladislav Curn

❮ Previous
Next ❯

Citations

May 4, 2021·Frontiers in Plant Science·Piotr M KopecWojciech M Karlowski

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
genotyping
electrophoresis

Software Mentioned

BCFtools
MapChart
WinQTL
SAMtools
Bowtie2
JoinMap
fastqc
IciMapping
BRAD
WinQTL cartographer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.