Quantitative zeptomolar imaging of miRNA cancer markers with nanoparticle assemblies

Proceedings of the National Academy of Sciences of the United States of America
Aihua QuHua Kuang

Abstract

Multiplexed detection of small noncoding RNAs responsible for posttranscriptional regulation of gene expression, known as miRNAs, is essential for understanding and controlling cell development. However, the lifetimes of miRNAs are short and their concentrations are low, which inhibits the development of miRNA-based methods, diagnostics, and treatment of many diseases. Here we show that DNA-bridged assemblies of gold nanorods with upconverting nanoparticles can simultaneously quantify two miRNA cancer markers, namely miR-21 and miR-200b. Energy upconversion in nanoparticles affords efficient excitation of fluorescent dyes via energy transfer in the superstructures with core-satellite geometry where gold nanorods are surrounded by upconverting nanoparticles. Spectral separation of the excitation beam and dye emission wavelengths enables drastic reduction of signal-to-noise ratio and the limit of detection to 3.2 zmol/ngRNA (0.11 amol or 6.5 × 104 copies) and 10.3 zmol/ngRNA (0.34 amol or 2.1 × 105 copies) for miR-21 and miR-200b, respectively. Zeptomolar sensitivity and analytical linearity with respect to miRNA concentration affords multiplexed detection and imaging of these markers, both in living cells and in vivo assays. The...Continue Reading

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Jul 10, 2019·Nucleic Acids Research·Arun Richard ChandrasekaranKen Halvorsen
Feb 29, 2020·Chemical Science·Xiao-Long ZhangRuo Yuan
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Methods Mentioned

BETA
FRET
LRET
biosensing
Fluorescence
confocal microscopy
transfection
xenografts

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