Quantity rather than quality in teratospermic males: a histomorphometric and flow cytometric evaluation of spermatogenesis in the domestic cat (Felis catus)

Biology of Reproduction
Katrin NeubauerBudhan Pukazhenthi

Abstract

Teratozoospermia (ejaculation of <40% morphologically normal sperm) commonly occurs within the Felidae, including certain domestic cats, but the cellular and molecular mechanisms that give rise to this phenomenon remain unknown. This study quantified spermatogenesis to identify differential dysfunctions in teratospermic versus normospermic (>60% normal sperm/ejaculate) domestic cats. Sperm used were from electroejaculates and cauda epididymides. Testes from 10 normo- and 10 teratospermic males were obtained by castration and then evaluated by histomorphometry, flow cytometry, and testicular testosterone enzyme immunoassay. Some morphometric traits (tubular diameter, epithelium height, interstitial area, number of Leydig cells, and blood vessels per cross-section) as well as testicular testosterone concentrations were similar between groups, but testicular volume was greater in teratospermic males. Stage frequencies differed also between both cat populations, suggesting possible dysfunctions in spermiation. Quantification of cell populations in most frequent stages revealed more spermatogenic cells and fewer Sertoli cells per tubule cross-section as well as per tissue unit in teratospermic donors. Hence, the ratio of spermatogen...Continue Reading

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Citations

Sep 9, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Natalia GañánEduardo R S Roldan
Nov 11, 2008·Theriogenology·J G Howard, D E Wildt
May 18, 2007·Reproduction in Domestic Animals = Zuchthygiene·E Axnér, C Linde Forsberg
Sep 12, 2007·Reproduction in Domestic Animals = Zuchthygiene·S Blottner, K Jewgenow
Jul 11, 2006·Journal of Anatomy·Estela Sasso-CerriSandra Maria Miraglia
Apr 20, 2006·Theriogenology·Gaia Cecilia Luvoni
Apr 29, 2006·Theriogenology·Budhan S PukazhenthiDavid E Wildt
May 21, 2015·ILAR Journal·Raquel González, Ina Dobrinski
May 4, 2012·Toxicologic Pathology·Emiko HaruyamaAkihide Tanimoto
Feb 9, 2012·Journal of Feline Medicine and Surgery·Karen V DiagoneWilter R R Vicente
Dec 13, 2012·Journal of Feline Medicine and Surgery·Ana Izabel S Balbin VillaverdeMaria Denise Lopes
Jul 3, 2020·Journal of Evolutionary Biology·Gabriela CardozoAndrea Pilastro
Sep 22, 2015·Reproduction, Fertility, and Development·Diana C KoesterAdrienne E Crosier
Jan 29, 2021·International Journal of Molecular Sciences·Rosana Rodríguez-Casuriaga, Adriana Geisinger
Feb 23, 2021·Reproduction in Domestic Animals = Zuchthygiene·Lucile BodetAnne Gogny
Mar 7, 2021·Animals : an Open Access Journal From MDPI·Sylwia ProchowskaWojciech Niżański
Oct 11, 2020·Theriogenology·Alain FontbonneZuzanna Niewiadomska

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

SPSS
DPAC
analySIS

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