Quantum Cascade Laser-Based Infrared Microscopy for Label-Free and Automated Cancer Classification in Tissue Sections

Scientific Reports
C KuepperK Gerwert

Abstract

A feasibility study using a quantum cascade laser-based infrared microscope for the rapid and label-free classification of colorectal cancer tissues is presented. Infrared imaging is a reliable, robust, automated, and operator-independent tissue classification method that has been used for differential classification of tissue thin sections identifying tumorous regions. However, long acquisition time by the so far used FT-IR-based microscopes hampered the clinical translation of this technique. Here, the used quantum cascade laser-based microscope provides now infrared images for precise tissue classification within few minutes. We analyzed 110 patients with UICC-Stage II and III colorectal cancer, showing 96% sensitivity and 100% specificity of this label-free method as compared to histopathology, the gold standard in routine clinical diagnostics. The main hurdle for the clinical translation of IR-Imaging is overcome now by the short acquisition time for high quality diagnostic images, which is in the same time range as frozen sections by pathologists.

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Citations

Mar 7, 2019·The Analyst·Shachi Mittal, Rohit Bhargava
Apr 13, 2019·Annual Review of Analytical Chemistry·Dale ChristensenBayden Wood
Nov 25, 2018·Optics Express·Jakob KilgusMarkus Brandstetter
Dec 20, 2019·Nucleic Acids Research·Lena Voith von VoithenbergGovind V Kaigala
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Nov 18, 2018·The Analyst·Katharina IsenseeWolfgang Petrich
Jun 18, 2020·Frontiers in Cell and Developmental Biology·Stéphane BrézillonGanesh D Sockalingum
Jun 25, 2020·Scientific Reports·Angela Kallenbach-ThieltgesKlaus Gerwert
Oct 16, 2018·Biomedical Optics Express·Yu-Pei TsengPeter Tidemand-Lichtenberg
Feb 6, 2020·Proceedings of the National Academy of Sciences of the United States of America·Martin SchnellRohit Bhargava
Oct 30, 2020·Nucleic Acids Research·Nikolaos-Kosmas ChlisFabian J Theis
Sep 29, 2018·Applied Spectroscopy·Susanne PahlowRohit Bhargava
Jan 4, 2019·Analytical Chemistry·Kevin YehRohit Bhargava
Sep 9, 2020·The Journal of Physical Chemistry Letters·Emanuel Pfitzner, Joachim Heberle
Dec 4, 2019·Analytical Chemistry·Rupali MankarDavid Mayerich
Dec 16, 2020·Analytical Chemistry·Yamuna PhalRohit Bhargava
Apr 21, 2021·Applied Spectroscopy·Yamuna PhalRohit Bhargava
May 28, 2021·Applied Spectroscopy·James M CameronMatthew J Baker
Oct 14, 2021·Applied Spectroscopy·Laurin LuxRohit Bhargava

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Methods Mentioned

BETA
imaging technique
laser capture microdissection
biopsies

Software Mentioned

Spero QT
Daylight

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