Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification

International Journal of Nanomedicine
Wei-Juan ZengYan Li

Abstract

The aims of this study are to establish a new method for simultaneously detecting the interactions between cancer cells and immunocytes in malignant ascites (MA) and to propose a new model for MA classification. A quantum dot (QD)-based multiplexed imaging technique was developed for simultaneous in situ imaging of cancer cells, lymphocytes, and macrophages. This method was first validated in gastric cancer tissues, and then was applied to MA samples from 20 patients with peritoneal carcinomatosis from gastrointestinal and gynecological origins. The staining features of MA and the interactions between cancer cells and immunocytes in the ascites were further analyzed and correlated with clinical features. The QD-based multiplexed imaging technique was able to simultaneously show gastric cancer cells, infiltrating macrophages, and lymphocytes in tumor tissue, and the technique revealed the distinctive features of the cancer tumor microenvironment. When this multiplexed imaging protocol was applied to MA cytology, different features of the interactions and quantitative relations between cancer cells and immunocytes were observed. On the basis of these features, MA could be classified into immunocyte-dominant type, immunocyte-react...Continue Reading

Citations

Sep 23, 2016·Chemical Reviews·Gaixia XuParas N Prasad
Jun 28, 2020·AAPS PharmSciTech·Laura CabezaConsolación Melguizo
Jul 17, 2018·Gastroenterology Research and Practice·Gianluca PellinoFrancesco Selvaggi

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