Quantum dot based rapid tests for zearalenone detection

Analytical and Bioanalytical Chemistry
N V BeloglazovaI Yu Goryacheva

Abstract

Three different kinds of immunosorbent assays with luminescence detection were developed for the determination of zearalenone (ZEN), a secondary toxic metabolite of Fusarium fungi. CdSe/ZnS core/shell quantum dots (QDs) were used as a label in quantitative micro-well plate immunoassays (fluorescent-labeled immunosorbent assay, FLISA) and in qualitative column test methods. As carriers for QD-based column tests, sepharose gel (for covalent binding of antibody) and polyethylene frits (for physical absorption of antibody) were used and compared. The application of QDs as a label resulted in a fourfold decrease in the IC(50) value with FLISA (0.1 ng mL(-1)) with a detection limit of 0.03 ng mL(-1) when compared with the traditional immunosorbent assay which makes use of horseradish peroxidase as the enzyme label. The cutoff levels for both qualitative column test methods were selected based on the maximum level for ZEN in unprocessed cereals established by the European Commission (100 μg kg(-1)) as 5 ng mL(-1) taking into account extraction and dilution. The different developed immumoassays were tested for ZEN determination in raw wheat samples. As a confirmatory method, liquid chromatography coupled to tandem mass spectrometry was...Continue Reading

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Citations

Oct 22, 2013·Biosensors & Bioelectronics·Elena S SperanskayaIrina Yu Goryacheva
Jun 22, 2013·Analytical Chemistry·N V BeloglazovaI Yu Goryacheva
May 9, 2014·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Qing WangShijia Ding
Jun 19, 2013·Analytical and Bioanalytical Chemistry·N V BeloglazovaS De Saeger
May 23, 2014·Analytical and Bioanalytical Chemistry·L AnfossiC Baggiani
May 9, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Herman S MansurMaria de Fatima Leite
Jan 7, 2015·Critical Reviews in Analytical Chemistry·Katarzyna KwaśniewskaKrzysztof Cendrowski
Jun 4, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Elena S SperanskayaZeger Hens
Dec 13, 2016·Journal of Agricultural and Food Chemistry·Astrid FoubertSarah De Saeger
Jan 11, 2017·Critical Reviews in Food Science and Nutrition·Pranav TripathiSeema Nara
Dec 27, 2016·Journal of Agricultural and Food Chemistry·Natalia V BeloglazovaSarah De Saeger
Jun 2, 2020·Analytical and Bioanalytical Chemistry·Han FuGuitang Chen
Nov 25, 2020·Sensors·Ming-Jer JengChao-Sung Lai
Jan 5, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Poorvisha Ravi, Muthupandian Ganesan
Dec 11, 2019·Talanta·Natalia A BurmistrovaIrina Yu Goryacheva
Jun 19, 2021·Biological Reviews of the Cambridge Philosophical Society·Sanni M A FärkkiläLeho Tedersoo
May 7, 2020·ACS Applied Materials & Interfaces·Olga A GoryachevaNikolai Gaponik

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