PMID: 19928184Nov 26, 2009Paper

Quantum dot labeling and imaging of glial fibrillary acidic protein intermediate filaments and gliosis in the rat neural retina and dissociated astrocytes

Journal of Nanoscience and Nanotechnology
Smita PathakGabriel A Silva

Abstract

The use of antibody and peptide functionalized semiconductor quantum dots holds considerable potential for specific labeling of target antigens and high resolution optical imaging of biological preparations. Despite this potential, their use in neuroscience is not yet widespread; a number of technical and methodological challenges must still be overcome in order to produce reliable and reproducible labeling protocols. We have optimized and used anti-GFAP functionalized quantum dots for specific labeling of intermediate filaments in astrocyte and Müller glial cells in sections of intact rat neural sensory retina and dissociated primary spinal cord astrocytes. These techniques produced stable and robust imaging of retinal astrocytes and Müller cells with minimal non-specific background labeling and intense fluorescence resulting in a high signal to noise ratio. This resulted in clear and efficient labeling of normal levels of GFAP in the retina and the ability to differentiate it from pathologically high levels of GFAP associated with reactive gliosis in a laser induced injury model. Labeling and imaging of dissociated astrocytes demonstrated the presence of what appeared to be highly complex organizations of fine intermediate fi...Continue Reading

Citations

Sep 5, 2002·Progress in Neurobiology·Vicente Felipo, Roger F Butterworth
Dec 3, 2010·Journal of Nanoscience and Nanotechnology·Betty R LiuHan-Jung Lee
Aug 27, 2019·Current Eye Research·Sidra SarwatMaitreyee Roy

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.