Quantum dots-based double imaging combined with organic dye imaging to establish an automatic computerized method for cancer Ki67 measurement

Scientific Reports
Lin-Wei WangJuan Liu

Abstract

As a widely used proliferative marker, Ki67 has important impacts on cancer prognosis, especially for breast cancer (BC). However, variations in analytical practice make it difficult for pathologists to manually measure Ki67 index. This study is to establish quantum dots (QDs)-based double imaging of nuclear Ki67 as red signal by QDs-655, cytoplasmic cytokeratin (CK) as yellow signal by QDs-585, and organic dye imaging of cell nucleus as blue signal by 4',6-diamidino-2-phenylindole (DAPI), and to develop a computer-aided automatic method for Ki67 index measurement. The newly developed automatic computerized Ki67 measurement could efficiently recognize and count Ki67-positive cancer cell nuclei with red signals and cancer cell nuclei with blue signals within cancer cell cytoplasmic with yellow signals. Comparisons of computerized Ki67 index, visual Ki67 index, and marked Ki67 index for 30 patients of 90 images with Ki67 ≤ 10% (low grade), 10% < Ki67 < 50% (moderate grade), and Ki67 ≥ 50% (high grade) showed computerized Ki67 counting is better than visual Ki67 counting, especially for Ki67 low and moderate grades. Based on QDs-based double imaging and organic dye imaging on BC tissues, this study successfully developed an automa...Continue Reading

References

Jun 28, 2005·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M ColozzaM J Piccart
Dec 14, 2006·Breast Cancer Research : BCR·Mark J BeresfordAndreas Makris
Apr 27, 2007·Virchows Archiv : an International Journal of Pathology·Ossama TawfikCarol J Fabian
Oct 24, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Lyndsay HarrisUNKNOWN American Society of Clinical Oncology
Mar 26, 2009·Applied Immunohistochemistry & Molecular Morphology : AIMM·Mark D GustavsonJason Christiansen
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Jun 29, 2011·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A GoldhirschUNKNOWN Panel members
Oct 1, 2011·Journal of the National Cancer Institute·Mitch DowsettUNKNOWN International Ki-67 in Breast Cancer Working Group
Mar 14, 2012·International Journal of Cancer. Journal International Du Cancer·Pericles PericleousEfstathios P Efstathopoulos
Aug 16, 2012·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Alexander C KlimowiczJoseph C Dort
Nov 10, 2013·Journal of the National Cancer Institute·Mei-Yin C PolleyUNKNOWN International Ki67 in Breast Cancer Working Group of the Breast International Group and North American Breast Cancer Group
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Jul 17, 2015·JAMA Oncology·UNKNOWN Global Burden of Disease Cancer CollaborationMohsen Naghavi

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Citations

Aug 31, 2016·Molecular Medicine Reports·Xia-Fei GengYan Li
Mar 25, 2021·Veterinary and Comparative Oncology·Antonella RigilloBarbara C Rütgen
Jun 24, 2021·Artificial Cells, Nanomedicine, and Biotechnology·Suhailah S Al-JameelAbdulhadi Baykal

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Methods Mentioned

BETA
xenograft
imaging technique
Fluorescence

Software Mentioned

CRi Nuance
SPSS

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