Quantum dots enhanced ultrasensitive detection of DNA adducts

Analytical Chemistry
Zhixin WangHailin Wang

Abstract

Here we demonstrate that quantum dots (QD) can greatly improve the ultrasensitive capillary electrophoresis-laser induced fluorescence immunoassay of trace anti-benzo(a)pyrene diol epoxide (BPDE)-DNA adducts from sensitivity to separation. We for the first time show that the target QD-antibody-DNA complex is not only effectively separated but also effectively focused by capillary electrophoresis. With the online laser-induced fluorescence detection coupled, the low limits of detection of 6.6 x 10(-21) mol in mass and 120 fM in concentration are achieved for BPDE-DNA adducts. The achieved ultrasensitivity allows for human exposure biomonitoring and shows promising applications of QD in various DNA analyses, including DNA damage.

References

Jun 9, 1983·Nature·D H Phillips
Oct 23, 2003·Nature Reviews. Cancer·Stephen S Hecht
Aug 2, 2005·Analytical Chemistry·Hailin WangX Chris Le
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Zhaohui FengMoon-shong Tang
Dec 29, 2007·Chemical Research in Toxicology·James A SwenbergRichard J Albertini
Feb 19, 2008·Analytical Chemistry·Glorimar Vicente, Luis A Colón
Apr 22, 2008·Nature Methods·Mark HowarthAlice Y Ting
Aug 30, 2008·Nature Methods·Ute Resch-GengerThomas Nann
Sep 24, 2008·Angewandte Chemie·Ron GillItamar Willner
Jun 19, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Feng FengHailin Wang
Jun 25, 2009·Nature Reviews. Cancer·Stephen S HechtDorothy K Hatsukami

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Citations

Jun 10, 2010·Analytical and Bioanalytical Chemistry·Megan K WagnerX Chris Le
Nov 28, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Stanley O AgboJussi V K Kukkonen
Jun 5, 2012·Journal of Environmental Sciences (China)·Zhilan ChenHailin Wang
May 7, 2011·Biomaterials·Shibin HeLijia Li
Jul 3, 2015·Biosensors & Bioelectronics·Xiaoqian ZhuShengfu Wang
Mar 9, 2012·Molecular and Cellular Biochemistry·Bipasha DeyVibha Rani
Mar 22, 2014·Electrophoresis·Maja StanisavljevicVojtech Adam
Jul 26, 2019·Cellular and Molecular Life Sciences : CMLS·Karolina BoguszewskaBolesław T Karwowski
May 26, 2010·Analytical Chemistry·Nicholas W FrostMichael T Bowser
Jun 15, 2010·Analytical Chemistry·Aleeta M PoweIsiah M Warner
Jan 17, 2013·The Journal of Physical Chemistry Letters·John C VinciLuis A Colón
Mar 14, 2013·Journal of the American Chemical Society·Shengquan LiuHailin Wang

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