Quasi-elastic light-scattering studies of single skeletal muscle fibers

Biophysical Journal
R C Haskell, F D Carlson

Abstract

Measurements were made of the intensity autocorrelation function, g(2)[tau], of light scattered from intact frog muscle fibers. During the tension plateau of an isometric tenanus, scattered field statistics were approximately Gaussian and intensity fluctuations were quasi-stationary. The half time, tau 1/2, for the decay of g(2)[tau] was typically 70 ms at a scattering angle of 30 degrees. The decay rate, 1/tau 1/2, of g(2)[tau] varied roughly linearly with the projection of the scattering vector on the fiber axis. 1/tau 1/2 was greater during the tension creep phase of tetani of highly stretched fibers, but was roughly independent of sarcomere length during the tension plateau. g(2)[tau] measured during rest or on diffraction pattern maxima during isometric contraction were flat with low amplitudes. These results are consistent with a model of a 200-mu m segment of an isometrically contracting fiber in which scattering material possesses relative axial velocities of 1-2 mu m/s accompanied by relative axial displacements greater than 0.1 mu m. The slow (1-2 mu m/s) motion of one portion of the fiber relative to another observed under the microscope (500X) during isometric contraction is consistent with the light-scattering resu...Continue Reading

Citations

Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Roberto BruzzoneHannah Monyer
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Ashkin, J M Dziedzic
Apr 1, 1990·Journal of Muscle Research and Cell Motility·Y YehM Jones

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