QUASIMODO 3 (QUA3) is a putative homogalacturonan methyltransferase regulating cell wall biosynthesis in Arabidopsis suspension-cultured cells.

Journal of Experimental Botany
Yansong MiaoLiwen Jiang

Abstract

Pectins are complex polysaccharides that are essential components of the plant cell wall. In this study, a novel putative Arabidopsis S-adenosyl-L-methionine (SAM)-dependent methyltransferase, termed QUASIMODO 3 (QUA3, At4g00740), has been characterized and it was demonstrated that it is a Golgi-localized, type II integral membrane protein that functions in methylesterification of the pectin homogalacturonan (HG). Although transgenic Arabidopsis seedlings with overexpression, or knock-down, of QUA3 do not show altered phenotypes or changes in pectin methylation, this enzyme is highly expressed and abundant in Arabidopsis suspension-cultured cells. In contrast, in cells subjected to QUA3 RNA interference (RNAi) knock-down there is less pectin methylation as well as altered composition and assembly of cell wall polysaccharides. Taken together, these observations point to a Golgi-localized QUA3 playing an essential role in controlling pectin methylation and cell wall biosynthesis in Arabidopsis suspension cell cultures.

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Citations

Dec 3, 2011·Proceedings of the National Academy of Sciences of the United States of America·Melani A AtmodjoDebra Mohnen
Mar 5, 2013·Annual Review of Plant Biology·Melani A AtmodjoDebra Mohnen
Nov 23, 2015·Journal of Experimental Botany·Charles T Anderson
Aug 3, 2013·The Plant Journal : for Cell and Molecular Biology·Hao WangLiwen Jiang
Feb 24, 2015·The Plant Journal : for Cell and Molecular Biology·Sang-Jin KimFederica Brandizzi
Jan 1, 2013·Plants·Jean-Claude MolletArnaud Lehner
Aug 18, 2017·Physiologia Plantarum·Moona RahikainenSaijaliisa Kangasjärvi
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Dec 17, 2020·Journal of Integrative Plant Biology·Baocai ZhangYihua Zhou
May 21, 2021·Development·Bruce D KohornSusan Kohorn
Oct 7, 2021·The Plant Cell·Ying Gu, Carolyn G Rasmussen

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Methods Mentioned

BETA
electron microscopy
transmission electron microscopy
PCR
transgenic
glycosylation

Software Mentioned

Adobe Photoshop
Primer
BLAST

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