Quasispecies dynamics of a hepatitis E virus 4 from the feces and liver biopsy of an acute hepatitis E patient during virus clearance

Journal of Medical Virology
Xue MeiZhigang Yi

Abstract

It has been documented the presence of quasispecies in hepatitis E virus (HEV) infection, whilst the implication of quasispecies in HEV-host interaction is poorly understood. Here we analyzed the whole genome sequences of a hepatitis E virus 4d from the feces and liver biopsy of a patient during icteric phase and convalescent phase in an acute hepatitis E infection. Viral RNAs were extracted, reversely transcribed and seven fragments encompassing the entire viral genome were amplified and cloned. By sequencing multiple colonies of each cloned viral genome amplicon with Sanger sequencing, we verified the existence of HEV quasispecies or intra-host genetic variations within the fecal and liver biopsy samples. There were broader genetic variations in the HEV ORF1 region including the PCP, HPX and RdRp regions during the convalescent phase whereas more genetic variations in the ORF2 P domain during the icteric phase. The quasispecies dynamics might reflect host immune pressure during the viral clearance. This article is protected by copyright. All rights reserved.

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