Quaternary structure and cleavage specificity of a poxvirus holliday junction resolvase

The Journal of Biological Chemistry
A D GarciaB Moss

Abstract

Recently, poxviruses were found to encode a protein with signature motifs present in the RuvC family of Holliday junction (HJ) resolvases, which have a key role in homologous recombination in bacteria. The vaccinia virus homolog A22 specifically cleaved synthetic HJ DNA in vitro and was required for the in vivo resolution of viral DNA concatemers into unit-length genomes with hairpin telomeres. It was of interest to further characterize a poxvirus resolvase in view of the low sequence similarity with RuvC, the absence of virus-encoded RuvA and RuvB to interact with, and the different functions of the viral and bacterial resolvases. Because purified A22 aggregated severely, studies were carried out with maltose-binding protein fused to A22 as well as to RuvC. Using gel filtration, chemical cross-linking, analytical ultracentrifugation, and light scattering, we demonstrated that A22 and RuvC are homodimers in solution. Furthermore, the dimeric form of the resolvase associated with HJ DNA, presumably facilitating the symmetrical cleavage of such structures. Like RuvC, A22 symmetrically cleaved fixed HJ junctions as well as junctions allowing strand mobility. Unlike RuvC and other members of the family, however, the poxvirus enzyme...Continue Reading

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C F GaronB Moss
Nov 25, 1989·Nucleic Acids Research·S M Kerr, G L Smith
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Shuman, B Moss
Oct 5, 1974·Journal of Molecular Biology·P Geshelin, K I Berns
Dec 1, 1981·Cell·R W Moyer, R L Graves
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·B M BaroudyB Moss
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·A SaitoH Shinagawa
Jul 20, 1993·Journal of Molecular Biology·C A Parsons, S C West
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·J SekiguchiS Shuman
Oct 29, 1997·Molecular and Cellular Biology·M F White, D M Lilley
May 26, 1998·Journal of Molecular Biology·M J Giraud-Panis, D M Lilley
Jun 14, 2000·The Journal of Biological Chemistry·A K Eggleston, S C West
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·A D GarciaB Moss
Sep 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Flavio Da Fonseca, Bernard Moss

❮ Previous
Next ❯

Citations

Nov 14, 2008·The Journal of Biological Chemistry·Matthew J CulybaFrederic D Bushman
Aug 6, 2011·BMC Molecular Biology·Václav BrázdaCheryl Arrowsmith
Jun 29, 2011·Virus Research·Ambra Lo PianoSilvia Ayora
Mar 13, 2012·Antiviral Research·Mark N Prichard, Earl R Kern
Apr 13, 2010·Journal of Molecular Biology·Matthew J CulybaFrederic D Bushman
Jun 20, 2006·Virology·Matthew J CulybaFrederic D Bushman
Sep 25, 2007·The Journal of Biological Chemistry·Matthew J CulybaFrederic D Bushman
Mar 26, 2016·The Journal of Biological Chemistry·Huiguang LiGregory D Van Duyne

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
A D GarciaB Moss
Current Opinion in Structural Biology
Anne-Cécile Déclais, David M J Lilley
Nature Reviews. Molecular Cell Biology
David M J Lilley, M F White
Proceedings of the National Academy of Sciences of the United States of America
A SaitoH Shinagawa
© 2021 Meta ULC. All rights reserved