Quercetin differently regulates insulin-mediated glucose transporter 4 translocation under basal and inflammatory conditions in adipocytes

Molecular Nutrition & Food Research
Manli XuFang Huang

Abstract

Quercetin is the most abundant dietary flavonol with beneficial regulation of glucose homeostasis, but its regulation of insulin action remains uncertain. This study aims to investigate the effects of quercetin on insulin-mediated glucose transporter 4 (GLUT4) translocation under basal and inflammatory conditions as well as the molecular mechanisms in adipocytes. The effects of quercetin on insulin-mediated GLUT4 translocation in 3T3-L1 cells under basal and insulin resistant conditions were investigated. Meanwhile, we investigated the effect of quercetin on AMP-activated protein kinase (AMPK) activation implicated in regulation of insulin action. Quercetin inhibited insulin-mediated GLUT4 translocation by inhibiting AS160 phosphorylation. Differently, when inflammatory challenge impaired insulin action in 3T3-L1 cells, quercetin inhibited IκB kinase β (IKKβ) phosphorylation and facilitated insulin signaling, leading to the restoration of insulin-mediated AS160 phosphorylation and downstream GLUT4 translocation. AMPK inhibitor Compound C or knockdown of AMPKα by small interfering RNA (siRNA) abolished both actions of quercetin. Results from mice adipose tissue (AT) further confirmed its positive regulation of AMPK phosphorylati...Continue Reading

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Citations

Jan 26, 2016·Journal of Dairy Science·Ann-Kathrin StoldtCornelia C Metges
Mar 16, 2016·Journal of Physiology and Biochemistry·Jarinyaporn NaowabootPatchareewan Pannangpetch
Sep 22, 2015·Frontiers in Physiology·Isabele B S GomesElisardo C Vasquez
Apr 20, 2016·International Journal of Molecular Sciences·Mohammed Kawser HossainSsang-Goo Cho
Aug 17, 2016·International Journal of Molecular Sciences·Thiago Melo Costa PereiraElisardo Corral Vasquez
Oct 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Su BuFuliang Cao
Oct 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Renate HaselgrüblerJulian Weghuber
Apr 28, 2019·International Journal of Molecular Sciences·Benedetta RussoSimona Frontoni

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