Quercetin induced apoptosis in association with death receptors and fludarabine in cells isolated from chronic lymphocytic leukaemia patients.

British Journal of Cancer
M RussoG-L Russo

Abstract

Quercetin is a flavonoid naturally present in food and beverages belonging to the large class of phytochemicals with potential anti-cancer properties. Here, we investigated the ability of quercetin to sensitise primary cells from chronic lymphocytic leukaemia (CLL) to death receptor (DR) agonists, recombinant TNF-related-apoptosis-inducing ligand (rTRAIL) and anti-CD95, and to fludarabine, a widely used chemotherapeutic drug against CLL. Peripheral white blood cells were isolated from patients and incubated with medium containing 50 ng ml anti-CD95 agonist antibody; 10 ng ml recombinant TRAIL; 10-25 microM quercetin and 3.5-14 microM fludarabine. Neutral Red assay was used to measure cell viability, where as apoptosis was assessed by determining caspase-3 activity, exposure to Annexin V and PARP fragmentation. Quercetin significantly enhanced anti-CD95- and rTRAIL-induced cell death as shown by decreased cell viability, increased caspase-3 and -9 activities, and positivity to Annexin V. In addition, association of quercetin with fludarabine increases the apoptotic response in CLL cells of about two-fold compared with quercetin monotreatment. This work shows that resistance to DR- and fludarabine-induced cell death in leukaemic ...Continue Reading

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Citations

Aug 25, 2011·European Heart Journal·Gian Luigi RussoEugenio Picano
May 10, 2011·Expert Opinion on Therapeutic Targets·Giorgio ZauliPaola Secchiero
Oct 16, 2015·Critical Reviews in Food Science and Nutrition·José C J M D S MenezesMarc Diederich
May 16, 2012·Nutritional Neuroscience·Claudio Giovannini, Roberta Masella
Dec 1, 2018·Journal of Cellular Physiology·Raedeh SaraeiAli Hassanzadeh
Jul 14, 2011·British Journal of Cancer·C SpagnuoloG L Russo
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Feb 15, 2014·Oncotarget·Christian Billard

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Methods Mentioned

BETA
density-gradient centrifugation

Software Mentioned

CellQuest

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