Quercetin modulates signaling pathways and induces apoptosis in cervical cancer cells

Bioscience Reports
Madhumitha Kedhari SundaramArif Hussain

Abstract

Cancer cells have the unique ability to overcome natural defense mechanisms, undergo unchecked proliferation and evade apoptosis. While chemotherapeutic drugs address this, they are plagued by a long list of side effects and have a poor success rate. This has spurred researchers to identify safer bioactive compounds that possess chemopreventive and therapeutic properties. A wide range of experimental as well as epidemiological data encourage the use of dietary agents to impede or delay different stages of cancer. In the present study, we have examined the anti-ancer property of ubiquitous phytochemical quercetin by using cell viability assay, flow cytometry, nuclear morphology, colony formation, scratch wound assay, DNA fragmentation and comet assay. Further, qPCR analysis of various genes involved in apoptosis, cell cycle regulation, metastasis and different signal transduction pathways was performed. Proteome profiler was used to quantitate the expression of several of these proteins. We find that quercetin decreases cell viability, reduces colony formation, promotes G2-M cell cycle arrest, induces DNA damage and encourages apoptosis. Quercetin induces apoptosis via activating both apoptotic pathways with a stronger effect of...Continue Reading

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Citations

Dec 12, 2019·Critical Reviews in Food Science and Nutrition·Dinh-Chuong PhamBharath Kumar Velmurugan
Aug 18, 2020·Assay and Drug Development Technologies·Farrah SollTyler C Moore
Oct 1, 2020·Biomolecules·Ebrahim Rahmani MoghadamAlan Prem Kumar
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Jan 13, 2021·Seminars in Cancer Biology·Madhumitha Kedhari SundaramArif Hussain
Feb 10, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lianghui ZhaoXianli Du
Feb 4, 2021·International Journal of Molecular Sciences·Marta WoźniakSiddarth Agrawal
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cristina Adriana DeheleanIulia Pinzaru
Feb 25, 2021·Drug Design, Development and Therapy·Wenbin XuXueqiong Zhu
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Saleh A AlmatroodiArshad Husain Rahmani
Jul 28, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardAbbas Basiri

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Methods Mentioned

BETA
fluorescence-activated cell sorter
electrophoresis
Assay
flow cytometry
X-ray
light microscopy
DNA fragmentation assay

Software Mentioned

UCSF Chimera
ImageLab
SwissDock
OpenComet
QuantStudio3

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