Quercetin Potentiates Docosahexaenoic Acid to Suppress Lipopolysaccharide-induced Oxidative/Inflammatory Responses, Alter Lipid Peroxidation Products, and Enhance the Adaptive Stress Pathways in BV-2 Microglial Cells.

International Journal of Molecular Sciences
Grace Y SunC Michael Greenlief

Abstract

High levels of docosahexaenoic acid (DHA) in the phospholipids of mammalian brain have generated increasing interest in the search for its role in regulating brain functions. Recent studies have provided evidence for enhanced protective effects when DHA is administered in combination with phytochemicals, such as quercetin. DHA and quercetin can individually suppress lipopolysaccharide (LPS)⁻induced oxidative/inflammatory responses and enhance the antioxidative stress pathway involving nuclear factor erythroid-2 related factor 2 (Nrf2). However, studies with BV-2 microglial cells indicated rather high concentrations of DHA (IC50 in the range of 60⁻80 µM) were needed to produce protective effects. To determine whether quercetin combined with DHA can lower the levels of DHA needed to produce protective effects in these cells is the goal for this study. Results showed that low concentrations of quercetin (2.5 µM), in combination with DHA (10 µM), could more effectively enhance the expression of Nrf2 and heme oxygenase 1 (HO-1), and suppress LPS⁻induced nitric oxide, tumor necrosis factor-α, phospho-cytosolic phospholipase A₂, reactive oxygen species, and 4-hydroxynonenal, as compared to the same levels of DHA or quercetin alone. Th...Continue Reading

References

Apr 18, 2008·European Journal of Pharmacology·Agnes W BootsAalt Bast
Feb 5, 2009·Neuromolecular Medicine·Albert Y SunGrace Y Sun
Mar 2, 2010·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Wooki KimRobert S Chapkin
Oct 20, 2010·The British Journal of Nutrition·Jeremy P E Spencer
Aug 16, 2011·The Journal of Nutritional Biochemistry·Cica VissiennonVeronika Butterweck
Dec 27, 2011·Chemico-biological Interactions·Ana Belén Granado-SerranoSonia Ramos
Jan 30, 2013·Journal of Neuroinflammation·Dennis Y ChuangGrace Y Sun
Jun 25, 2013·Journal of Physiology and Biochemistry·Silvia Lorente-CebriánMaría J Moreno-Aliaga
Jan 31, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Meijuan ZhangFeng Zhang
Mar 7, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gulnaz BegumDandan Sun
May 18, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiri MlcekJiri Sochor
Aug 17, 2016·CNS & Neurological Disorders Drug Targets·Davide BarrecaSeyed Mohammad Nabavi
Mar 21, 2017·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Grace Y SunDavid Q Beversdorf
Apr 4, 2017·Journal of Nutritional Science and Vitaminology·Yasue Hosoyamada, Masako Yamada
Jun 13, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hye-Yoon BangYoung-Joon Surh
Aug 2, 2017·Biochemical and Biophysical Research Communications·Fuxing DongRuiqin Yao
Oct 17, 2017·European Journal of Pharmacology·Richard J Wurtman
Oct 17, 2017·Journal of Traditional and Complementary Medicine·Edwin L Cooper, Melissa J Ma
Oct 28, 2018·The European Journal of Neuroscience·Sanjay AryalSvend Davanger

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Citations

Nov 20, 2019·Current Pharmaceutical Design·David Q BeversdorfJudy Van de Water
Jun 5, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ou QiaoWenyuan Gao
Aug 14, 2020·Journal of Agricultural and Food Chemistry·Masuko KoboriToshiyuki Kimura

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Methods Mentioned

BETA
ELISA
protein assay
electrophoresis

Software Mentioned

MassLynx
QuantityOne
GraphPad Prism

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