Quercetin protects human granulosa cells against oxidative stress via thioredoxin system

Reproductive Biology
Zahra RashidiFardin Amidi

Abstract

Granulosa Cells (GCs) are sensitive to excessive production of reactive oxygen species (ROS). Quercetin (QUR) is a free radical scavenger which can alleviate oxidative stress through nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/antioxidant response element (ARE) pathway and thioredoxin (Trx) system. We aimed to explore the probable protective role of QUR on cultured human GCs treated with hydrogen peroxide (H2O2) as an inducer of oxidative stress. MTT assay was applied for evaluating the cell cytotoxicity of QUR and H2O2. The rate of apoptotic cells and intracellular ROS generation were determined by Annexin V-FITC/PI staining and 2'-7'-dichlorodihydrofluorescein diacetate fluorescent probes (DCFH-DA), respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis and western blot analysis were used to evaluate the gene and protein expression of Nrf2 and kelch-like ech-associated protein 1 (Keap1)1. The Nrf2 and Trx activities were measured by Enzyme-linked Immunosorbent Assay (ELISA). The results indicated that QUR pretreatment can decrease ROS production and apoptosis induced by H2O2. In addition, QUR increased Nrf2 gene and protein expression, as well as its nuclear translocation. Moreover, in QUR-trea...Continue Reading

References

Sep 13, 2003·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Ana ClaveroLuis Martinez
Aug 30, 2005·Toxicology and Applied Pharmacology·Mario AlíaLuis Goya
Apr 28, 2007·Free Radical Biology & Medicine·Shunsuke TanigawaDe-Xing Hou
Jul 24, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Zhen-Guo ChenYu-Cai Fu
Jul 27, 2010·The Journal of Nutritional Biochemistry·Ute BoettlerDoris Marko
Oct 29, 2011·Biology of Reproduction·Patrick J DevineUlrike Luderer
Dec 27, 2011·Chemico-biological Interactions·Ana Belén Granado-SerranoSonia Ramos
Jan 3, 2013·Systems Biology in Reproductive Medicine·Narendra Babu KaruputhulaKoel Chaudhury
Jun 6, 2013·Oxidative Medicine and Cellular Longevity·Borut PoljsakIrina Milisav
Jan 7, 2014·Vitamins and Hormones·Ulrike Luderer
Jan 11, 2014·Frontiers in Immunology·Eiji YoshiharaJunji Yodoi
May 6, 2014·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Meryem GencerVolkan Hancı
Jan 21, 2015·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Marcela CapcarovaAlexander V Sirotkin
Feb 24, 2015·European Journal of Pharmacology·Xin-Ting HuChen Xu
Apr 22, 2015·Molecules and Cells·Yoon-Jin LeeSang-Han Lee
Sep 26, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Xiaofang SunDaolin Tang
Feb 24, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Victor Udo NnaDaniel Udofia Owu
Jul 18, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ekramy ElmorsySaad Amer
Oct 19, 2017·Journal of Cellular Physiology·Michele AndreucciAshour Michael

❮ Previous
Next ❯

Citations

Jul 21, 2020·Journal of Food Biochemistry·Amirhossein DavoodvandiSadegh Jafarnejad
Jun 20, 2020·Phytotherapy Research : PTR·Zahra RashidiFardin Amidi
Oct 30, 2020·Antioxidants & Redox Signaling·Junmin ZhangJianguo Fang
Feb 12, 2021·Frontiers in Pharmacology·Liuqing YangQin Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis