Quo vadis? Microbial profiling revealed strong effects of cleanroom maintenance and routes of contamination in indoor environments

Scientific Reports
Christine Moissl-EichingerGabriele Berg

Abstract

Space agencies maintain highly controlled cleanrooms to ensure the demands of planetary protection. To study potential effects of microbiome control, we analyzed microbial communities in two particulate-controlled cleanrooms (ISO 5 and ISO 8) and two vicinal uncontrolled areas (office, changing room) by cultivation and 16S rRNA gene amplicon analysis (cloning, pyrotagsequencing, and PhyloChip G3 analysis). Maintenance procedures affected the microbiome on total abundance and microbial community structure concerning richness, diversity and relative abundance of certain taxa. Cleanroom areas were found to be mainly predominated by potentially human-associated bacteria; archaeal signatures were detected in every area. Results indicate that microorganisms were mainly spread from the changing room (68%) into the cleanrooms, potentially carried along with human activity. The numbers of colony forming units were reduced by up to ~400 fold from the uncontrolled areas towards the ISO 5 cleanroom, accompanied with a reduction of the living portion of microorganisms from 45% (changing area) to 1% of total 16S rRNA gene signatures as revealed via propidium monoazide treatment of the samples. Our results demonstrate the strong effects of cl...Continue Reading

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Citations

Sep 18, 2015·Frontiers in Microbiology·Alexander MahnertGabriele Berg
Oct 27, 2017·Microbiome·Kaisa KoskinenChristine Moissl-Eichinger
Sep 17, 2019·Obstetric Medicine·Sam SchoenmakersMarijke Faas
Jun 30, 2016·FEMS Microbiology Reviews·Christine Moissl-EichingerPetra Rettberg
Dec 20, 2018·Frontiers in Microbiology·Gustavo A RamírezSteven D'Hondt

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Datasets Mentioned

BETA
JQ855509-635

Methods Mentioned

BETA
454
PMA
PCR
chips
as

Software Mentioned

BioTyper
uclust
mothur
PhyloChip
Cytoscape
PyNAST
ChimeraSlayer
R
QIIME

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