Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones.

Microbiology
Kay H McCleanPaul Williams

Abstract

Quorum sensing relies upon the interaction of a diffusible signal molecule with a transcriptional activator protein to couple gene expression with cell population density. In Gram-negative bacteria, such signal molecules are usually N-acylhomoserine lactones (AHLs) which differ in the structure of their N-acyl side chains. Chromobacterium violaceum, a Gram-negative bacterium commonly found in soil and water, produces the characteristic purple pigment violacein. Previously the authors described a violacein-negative, mini-Tn5 mutant of C. violaceum (CV026) in which pigment production can be restored by incubation with supernatants from the wild-type strain. To develop this mutant as a general biosensor for AHLs, the natural C. violaceum AHL molecule was first chemically characterized. By using solvent extraction, HPLC and mass spectrometry, a single AHL, N-hexanoyl-L-homoserine lactone (HHL), was identified in wild-type C. violaceum culture supernatants which was absent from CV026. Since the production of violacein constitutes a simple assay for the detection of AHLs, we explored the ability of CV026 to respond to a series of synthetic AHL and N-acylhomocysteine thiolactone (AHT) analogues. In CV026, violacein is inducible by all...Continue Reading

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Citations

Dec 1, 2006·Archives of Microbiology·Stéphane UrozYves Dessaux
Apr 15, 2008·Analytical and Bioanalytical Chemistry·Anjali KumariSylvia Daunert
Sep 19, 2012·Analytical and Bioanalytical Chemistry·Tommaso R I CataldiPhilippe Schmitt-Kopplin
Mar 14, 2007·Microbial Ecology·Torben MartensThorsten Brinkhoff
May 27, 2010·Microbial Ecology·Mariella O RivasCarlos E Riquelme
May 27, 2011·Microbial Ecology·Maria-Luisa Gutierrez-ZamoraMike Manefield
Feb 15, 2011·Applied Microbiology and Biotechnology·Joanne HothersallChristopher M Thomas
Jun 29, 2007·Current Microbiology·Gulgun Bosgelmez-TinazOzgur Ceylan
Sep 4, 2009·Current Microbiology·Jishun LuYuquan Xu
Jun 15, 2010·Current Microbiology·Kok-Gan ChanKek-Heng Chua
Dec 18, 2010·Molecular Genetics and Genomics : MGG·Eiko KandaYuki Ichinose
May 4, 2012·Molecular Genetics and Genomics : MGG·Yuichiro KawakitaYuki Ichinose
May 24, 2005·Extremophiles : Life Under Extreme Conditions·Inmaculada LlamasJuan E González
Sep 14, 2010·Extremophiles : Life Under Extreme Conditions·Craig Baker-AustinMark Dopson
Oct 24, 2008·Journal of Industrial Microbiology & Biotechnology·Kok-Gan ChanChong-Lek Koh
Apr 20, 2006·Antonie van Leeuwenhoek·Inmaculada LlamasEmilia Quesada
Jun 1, 2012·Journal of Chemical Ecology·Anton Hartmann, Adam Schikora
Jun 1, 2011·World Journal of Microbiology & Biotechnology·Saeed Tarighi, Parissa Taheri
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