Quorum sensing and motility mediate interactions between Pseudomonas aeruginosa and Agrobacterium tumefaciens in biofilm cocultures

Proceedings of the National Academy of Sciences of the United States of America
Dingding AnMatthew R Parsek

Abstract

In the environment, multiple microbial taxa typically coexist as communities, competing for resources and, often, physically associated within biofilms. A dual-species cocultivation model has been developed by using two ubiquitous and well studied microbes Pseudomonas aeruginosa (P.a.) and Agrobacterium tumefaciens (A.t.) as a tractable system to identify molecular mechanisms that underlie multispecies microbial associations. Several factors were found to influence coculture interactions. P.a. had a distinct growth-rate advantage in cocultures, increasing its relative abundance during planktonic and biofilm growth. P.a. also demonstrated a slight quorum-sensing-dependent increase in growth yield in liquid cocultures. P.a. dominated coculture biofilms, "blanketing" or burying immature A.t. microcolonies. P.a. flagellar and type IV pili mutant strains exhibited deficient blanketing and impaired competition in coculture biofilms, whereas, in planktonic coculture, these mutations had no effect on competition. In contrast, A.t. used motility to emigrate from coculture biofilms. In both planktonic and biofilm cocultures, A.t. remained viable for extended periods of time, coexisting with its more numerous competitor. These findings re...Continue Reading

References

Jan 1, 1995·Annual Review of Microbiology·J W CostertonH M Lappin-Scott
Nov 5, 1999·Methods in Enzymology·B B ChristensenS Molin
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·M R Parsek, E P Greenberg
Sep 7, 2001·Annual Review of Microbiology·M B Miller, B L Bassler
Oct 10, 2001·Journal of Bacteriology·L A Gallagher, C Manoil
Jun 12, 2002·Journal of Bacteriology·Scott A BeatsonJohn S Mattick
Jul 27, 2002·Annual Review of Microbiology·John S Mattick
Apr 5, 2003·Applied and Environmental Microbiology·Gail M Teitzel, Matthew R Parsek
Jun 7, 2003·Molecular Microbiology·Mikkel KlausenTim Tolker-Nielsen
Sep 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michiko E Taga, Bonnie L Bassler
Nov 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Huimin RanGee W Lau
Dec 9, 2003·Applied and Environmental Microbiology·C SchmeisserW R Streit
Jul 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·David Tilman
Sep 3, 2004·Molecular Microbiology·Lian-Hui Zhang, Yi-Hu Dong
Nov 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Paul G EglandPaul E Kolenbrander
Nov 24, 2004·Molecular Microbiology·Deborah A HoganRoberto Kolter
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J TempéJ Schell

❮ Previous
Next ❯

Citations

Nov 13, 2008·Journal of Industrial Microbiology & Biotechnology·S P Galíndez-NájeraJ Galíndez-Mayer
Feb 3, 2012·Environmental Science & Technology·Joshua D Shrout, Robert Nerenberg
May 20, 2006·Nature·Roberto Kolter, E Peter Greenberg
Jul 6, 2012·The ISME Journal·Josephine R ChandlerE Peter Greenberg
Dec 1, 2009·Nature Reviews. Microbiology·Michael E HibbingS Brook Peterson
Oct 24, 2008·Environmental Technology·S M Tiquia
Sep 13, 2008·Molecular Plant-microbe Interactions : MPMI·Carmen M HerreraSusanne B von Bodman
Aug 14, 2009·Journal of the Royal Society, Interface·Steve Atkinson, Paul Williams
Jun 15, 2007·Applied and Environmental Microbiology·Katharina BesemerTom J Battin
Sep 29, 2009·Applied and Environmental Microbiology·Dhritiman GhoshMark Radosevich
Apr 17, 2007·Applied and Environmental Microbiology·Changhui GuanJo Handelsman
May 29, 2007·Applied and Environmental Microbiology·Taketo KawaraiMakari Yamasaki
Jun 8, 2011·Infection and Immunity·Matthew S ByrdW Edward Swords
Sep 4, 2007·Journal of Bacteriology·Peter M MerrittClay Fuqua
Sep 11, 2012·Journal of Bacteriology·Robert J C McLeanLori L Graham
Jun 3, 2009·Microbiology and Molecular Biology Reviews : MMBR·Ece Karatan, Paula Watnick
Dec 7, 2007·Canadian Journal of Microbiology·Youai HaoBernard R Glick
Jun 12, 2008·Annual Review of Microbiology·Ainslie E F LittleJo Handelsman
Nov 13, 2010·Bioscience, Biotechnology, and Biochemistry·Soichi FurukawaYasushi Morinaga
Sep 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kelsi M SandozMartin Schuster
Aug 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jaione ValleJean-Marc Ghigo
Dec 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lucas R HoffmanSamuel I Miller
Sep 5, 2009·Applied Microbiology and Biotechnology·Robin Tecon, Jan Roelof van der Meer
Jan 1, 2012·Journal of Environmental Sciences (China)·Siqing XiaJixiang Li
Feb 22, 2008·Trends in Microbiology·Marc Ongena, Philippe Jacques
Nov 7, 2015·Microbial Pathogenesis·Hong Min NgEric C Reynolds
Jun 11, 2009·Environmental Microbiology·Karen TaitIan Joint
Oct 10, 2006·Cellular Microbiology·Mary Jo Kirisits, Matthew R Parsek

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