Quorum sensing modulation of a putative glycosyltransferase gene cluster essential for Xanthomonas campestris biofilm formation

Environmental Microbiology
Fei TaoLian-Hui Zhang

Abstract

Findings from previous studies suggest that the quorum sensing signal DSF (diffusible signal factor) negatively regulates biofilm formation in Xanthomonas campestris pv. campestris (Xcc) by affecting the expression of manA encoding biofilm dispersion and an unknown factor(s). In this study, by analysing the double deletion mutant ΔrpfFΔmanA, in which DSF biosynthesis gene rpfF and biofilm dispersal gene manA were deleted, we found that DSF modulated biofilm development by suppression of a mechanism essential for biofilm formation. Transposon mutagenesis of ΔrpfFΔmanA and subsequent analyses led to the identification of a novel gene locus xagABC encoding a putative glycosyl transferase system. Genetic analysis revealed that the transcriptional expression of xagABC was negatively regulated by DSF through the RpfC/RpfG two-component regulatory system. Deletion of the xag genes resulted in decreased extracellular polysaccharide production, abolished Xcc biofilm formation and attenuated the bacterial resistance to oxidative stress. Furthermore, we provide evidence that xagABC and manA were differentially expressed in Xcc and the biofilm formed by overexpression of xagABC in wild-type Xcc could be dispersed by ManA. These results pro...Continue Reading

References

Feb 13, 2001·Trends in Microbiology·T F Mah, G A O'Toole
Apr 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Hai-Bao ZhangLian-Hui Zhang
Apr 5, 2002·Clinical Microbiology Reviews·Rodney M Donlan, J William Costerton
Jun 1, 1994·Plant Physiology·M. C. Mehdy
May 6, 2003·Annual Review of Phytopathology·Cindy E Morris, Jean-Michel Monier
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·J Maxwell DowJi-Liang Tang
Jan 21, 2004·Molecular Microbiology·Lian-Hui WangLian-Hui Zhang
Feb 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Karyn L NewmanSteven E Lindow
Jun 12, 2004·Plant Physiology and Biochemistry : PPB·Ricardo CaponeAlex Levine
Jan 11, 2005·Trends in Microbiology·Luanne Hall-Stoodley, Paul Stoodley
Jan 22, 2005·Molecular Microbiology·Hemantha D KulasekaraStephen Lory
Jul 23, 2005·Glycobiology·Christelle BretonAnne Imberty
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Dingding AnMatthew R Parsek
May 18, 2007·Annual Review of Microbiology·Thomas Danhorn, Clay Fuqua
Jun 19, 2008·FEMS Microbiology Reviews·Ya-Wen He, Lian-Hui Zhang
Jan 6, 2009·The Plant Journal : for Cell and Molecular Biology·Diego SegondDominique Expert

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Citations

Dec 21, 2013·Water Research·Huajun FengJiali Shentu
Feb 2, 2016·Current Opinion in Microbiology·Olga E Petrova, Karin Sauer
Jan 14, 2011·Trends in Microbiology·Robert P Ryan, J Maxwell Dow
Mar 25, 2014·Current Opinion in Microbiology·Cristina SolanoIñigo Lasa
Aug 12, 2016·Nature Reviews. Microbiology·Kai Papenfort, Bonnie L Bassler
Nov 5, 2016·Research in Microbiology·Zhiwei SongFengquan Liu
Feb 6, 2017·Environmental Technology·Yangcheng DingYuyang Zhou
Apr 20, 2017·Molecular Microbiology·Cyril GuilhenDamien Balestrino
Mar 31, 2017·Critical Reviews in Microbiology·Hyun-Sul JungMarleen M Kock
Mar 6, 2018·Journal of Basic Microbiology·Yu-Fan LiuYi-Min Hsiao
Feb 4, 2012·Critical Reviews in Microbiology·H M H N BandaraLakshman Samaranayake
Jun 14, 2020·Nature Reviews. Microbiology·Kendra P Rumbaugh, Karin Sauer
Nov 3, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Joey R BernhardtAndrew Gonzalez
Jan 30, 2021·Computational and Structural Biotechnology Journal·Martin Zwanzig
Dec 21, 2010·Chemical Reviews·Yinyue DengLian-Hui Zhang
Feb 3, 2012·Environmental Science & Technology·Joshua D Shrout, Robert Nerenberg
Aug 17, 2021·Frontiers in Microbiology·Veronica LazarMariana Carmen Chifiriuc

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