R-Smad competition controls activin receptor output in Drosophila.

PloS One
Aidan J PetersonM B O'Connor

Abstract

Animals use TGF-β superfamily signal transduction pathways during development and tissue maintenance. The superfamily has traditionally been divided into TGF-β/Activin and BMP branches based on relationships between ligands, receptors, and R-Smads. Several previous reports have shown that, in cell culture systems, "BMP-specific" Smads can be phosphorylated in response to TGF-β/Activin pathway activation. Using Drosophila cell culture as well as in vivo assays, we find that Baboon, the Drosophila TGF-β/Activin-specific Type I receptor, can phosphorylate Mad, the BMP-specific R-Smad, in addition to its normal substrate, dSmad2. The Baboon-Mad activation appears direct because it occurs in the absence of canonical BMP Type I receptors. Wing phenotypes generated by Baboon gain-of-function require Mad, and are partially suppressed by over-expression of dSmad2. In the larval wing disc, activated Baboon cell-autonomously causes C-terminal Mad phosphorylation, but only when endogenous dSmad2 protein is depleted. The Baboon-Mad relationship is thus controlled by dSmad2 levels. Elevated P-Mad is seen in several tissues of dSmad2 protein-null mutant larvae, and these levels are normalized in dSmad2; baboon double mutants, indicating that ...Continue Reading

References

Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R W PadgettW M Gelbart
Oct 6, 1997·The EMBO Journal·A NakaoP ten Dijke
Jan 28, 1999·The Journal of Biological Chemistry·Y G Chen, J Massagué
Jun 18, 2003·Cell·Yigong Shi, Joan Massagué
Jun 4, 2004·Molecular Cell·Liangjun WangRichard S Jones
Feb 10, 2007·Molecular Cell·Gopal SapkotaJoan Massagué
Aug 23, 2008·Nature·Mansi SrivastavaDaniel S Rokhsar
Sep 30, 2008·Fly·Scott C Gesualdi, Theodor E Haerry
Dec 31, 2008·Cell Research·Ying E Zhang
Feb 5, 2009·BMC Evolutionary Biology·Lukasz HuminieckiCarl-Henrik Heldin
Feb 20, 2009·The Journal of Biological Chemistry·Katharine H WrightonXin-Hua Feng
Mar 18, 2009·Developmental Cell·Mary Y Wu, Caroline S Hill
Nov 1, 2007·Integrative and Comparative Biology·Vicki Buchsbaum Pearse, Oliver Voigt

❮ Previous
Next ❯

Citations

May 24, 2017·Development·Hrvoje AugustinLinda Partridge
Jul 28, 2017·Nature Communications·Kalpana MakhijaniKatja Brückner
Mar 1, 2018·Scientific Reports·Makoto I KanaiHiroshi Nakato
Jan 10, 2020·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Jia GouHans G Othmer
Jul 8, 2020·ELife·Ambuj UpadhyayMichael B O'Connor
Jan 29, 2017·Cold Spring Harbor Perspectives in Biology·Ambuj UpadhyayMichael B O'Connor
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Arpan C Ghosh, Michael B O'Connor
May 8, 2013·The Journal of Biological Chemistry·Masayo Sakaki-YumotoRik Derynck
Oct 26, 2018·Frontiers in Neuroscience·Xiaojuan SuDezhi Mu
May 11, 2012·Journal of Cell Science·Rebecca B SmithGuillermo Marqués

❮ Previous
Next ❯

Datasets Mentioned

BETA
AF101386

Methods Mentioned

BETA
confocal microscopy
PCR
immunoprecipitation
transfection
electrophoresis
Infrared Imaging
light microscopy

Software Mentioned

Axiovision
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.