R11: a cis-regulatory node of the sea urchin embryo gene network that controls early expression of SpDelta in micromeres

Developmental Biology
Roger Revilla-i-DomingoEric H Davidson

Abstract

A gene regulatory network (GRN) controls the process by which the endomesoderm of the sea urchin embryo is specified. In this GRN, the program of gene expression unique to the skeletogenic micromere lineage is set in train by activation of the pmar1 gene. Through a double repression system, this gene is responsible for localization of expression of downstream regulatory and signaling genes to cells of this lineage. One of these genes, delta, encodes a Notch ligand, and its expression in the right place and time is crucial to the specification of the endomesoderm. Here we report a cis-regulatory element R11 that is responsible for localizing the expression of delta by means of its response to the pmar1 repression system. R11 was identified as an evolutionarily conserved genomic sequence located about 13 kb downstream of the last exon of the delta gene. We demonstrate here that this cis-regulatory element is able to drive the expression of a reporter gene in the same cells and at the same time that the endogenous delta gene is expressed, and that temporally, spatially, and quantitatively it responds to the pmar1 repression system just as predicted for the delta gene in the endomesoderm GRN. This work illustrates the application o...Continue Reading

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Citations

Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Veronica F Hinman, Eric H Davidson
Aug 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·R Andrew CameronEric H Davidson
Mar 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Michael Levine, Eric H Davidson
Mar 26, 2005·BMC Bioinformatics·C Titus BrownR Andrew Cameron
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Dec 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Joel Smith, Eric H Davidson
Apr 21, 2009·The International Journal of Developmental Biology·Isabelle S Peter, Eric H Davidson
Oct 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Joel Smith, Eric H Davidson
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jongmin NamEric H Davidson
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Smadar Ben-Tabou de-Leon, Eric H Davidson
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Jun 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·Gregory A CaryVeronica F Hinman

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