R2R3-MYB transcription factor PpMYB17 positively regulates flavonoid biosynthesis in pear fruit.

Planta
Apekshika T PremathilakeYuanwen Teng

Abstract

PpMYB17 positively regulates flavonoid biosynthesis in pear fruit by activating PpCHS, PpCHI, PpF3H, and PpFLS in the flavonoid biosynthesis pathway independently of bHLH or WD40 cofactors in the MBW complex. Flavonoids are important secondary metabolites in plants. The flavonoid biosynthesis pathway is regulated by various transcription factors, with MYB transcription factors considered to be the key regulators. However, the regulation of flavonoid biosynthesis in the pear fruit has not been fully characterized. The R2R3-MYB transcription factor PpMYB17 was isolated from 'Red Zaosu' pear fruit and functionally characterized. An exposure to light upregulated PpMYB17 expression in the pear fruit. A phylogenetic analysis indicated PpMYB17 is related to the flavonol regulators. A subcellular localization assay suggested that PpMYB17 is a nuclear protein. Overexpression of PpMYB17 increased the flavonoid content of pear calli and Arabidopsis via the upregulated expression of structural genes in the flavonoid biosynthesis pathway, especially FLS. The LC-MS/MS analysis revealed most of the differentially accumulated flavonols, flavanones, flavones, isoflavones, and anthocyanins were significantly more abundant in PpMYB17-overexpressi...Continue Reading

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Citations

Dec 23, 2020·Journal of Plant Physiology·Shenghui JiangXuesen Chen

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Datasets Mentioned

BETA
LOC103967156

Methods Mentioned

BETA
transgenic
Assay
two-hybrid
one-hybrid

Software Mentioned

Analyst
MEGA
DNAMAN
SPSS

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