RAB-6.1 and RAB-6.2 Promote Retrograde Transport in C. elegans

PloS One
Donglei ZhangChristopher Rongo

Abstract

Retrograde transport is a critical mechanism for recycling certain membrane cargo. Following endocytosis from the plasma membrane, retrograde cargo is moved from early endosomes to Golgi followed by transport (recycling) back to the plasma membrane. The complete molecular and cellular mechanisms of retrograde transport remain unclear. The small GTPase RAB-6.2 mediates the retrograde recycling of the AMPA-type glutamate receptor (AMPAR) subunit GLR-1 in C. elegans neurons. Here we show that RAB-6.2 and a close paralog, RAB-6.1, together regulate retrograde transport in both neurons and non-neuronal tissue. Mutants for rab-6.1 or rab-6.2 fail to recycle GLR-1 receptors, resulting in GLR-1 turnover and behavioral defects indicative of diminished GLR-1 function. Loss of both rab-6.1 and rab-6.2 results in an additive effect on GLR-1 retrograde recycling, indicating that these two C. elegans Rab6 isoforms have overlapping functions. MIG-14 (Wntless) protein, which undergoes retrograde recycling, undergoes a similar degradation in intestinal epithelia in both rab-6.1 and rab-6.2 mutants, suggesting a broader role for these proteins in retrograde transport. Surprisingly, MIG-14 is localized to separate, spatially segregated endosomal ...Continue Reading

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Citations

Jun 20, 2017·Small GTPases·Angelika Hausser, Katalin Schlett
Jan 23, 2019·Journal of Cell Science·Jonathan D KimRachid El Bejjani
Feb 9, 2020·International Journal of Molecular Sciences·Sonya NassariSteve Jean

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Datasets Mentioned

BETA
CAG46781.1
AAF73841.1
AAF61637.1
CAG38500.1

Methods Mentioned

BETA
GTPase
GTPases
transgenic
ubiquitination
confocal microscopy
light microscopy
PCR

Software Mentioned

ClustalW
iVision
ImageJ

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