DOI: 10.1101/508598Dec 31, 2018Paper

Rab converter DMon1 constitutes a novel node in the brain-gonad axis essential for female germline maturation

BioRxiv : the Preprint Server for Biology
Neena DhimanAnuradha Ratnaparkhi


Monensin-sensitive 1 (Mon1) is an endocytic regulator that participates in the conversion of Rab5 positive early endosomes to Rab7 positive late endosomes. In Drosophila , loss of mon1 ( Dmon1 ) leads to sterility. The Dmon1 mutant females have extremely small ovaries with complete absence of late stage egg chambers, a phenotype reminiscent of mutations in the insulin pathway genes. Consistently, we find that expression of many Drosophila insulin-like peptides (Dilps) is down regulated in Dmon1 mutants. Conversely, feeding an insulin-rich diet can rescue the ovarian defects induced by the loss of Dmon1. Surprisingly however, Dmon1 is required in the tyramine/octopaminergic neurons (OPNs) and not in the ovaries or the insulin producing cells (IPCs). Thus, knockdown of Dmon1 in just the OPNs is sufficient to mimic the ovarian phenotype while expression of Dmon1 in the OPNs alone, is sufficient to rescue the mutant defect. Lastly, we have identified dilp5 as a critical target of Dmon1. Both, protein and mRNA levels of Dilp5 are reduced in Dmon1 mutants and IPC-specific dilp5 over expression can ameliorate the Dmon1 dependent sterility defect. The study thus identifies Dmon1 as a novel molecular player in the brain-gonad axis and u...Continue Reading

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