Rab11 Functions as an Oncoprotein via Nuclear Factor kappa B (NF-κB) Signaling Pathway in Human Bladder Carcinoma

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Xue GongPing Wang

Abstract

BACKGROUND Elevated expression of Rab11 has been reported in different human cancers, including human bladder carcinoma. This study, we investigated the biological effects and mechanism of Rab11 overexpression in human bladder carcinoma for the first time. MATERIAL AND METHODS Rab11 expression in bladder cancer tissues was detected using immunohistochemistry and Western blot analysis. Then, Rab11 expression was inhibited in T24 cells and it was overexpressed in BIU-87 cells. The effects of Rab11 perturbations on cell growth rate and invasion were analyzed by CCK8, cell cycle assay, and matrix gel invasion assay. MMP-9, cyclin E, and cyclin D1 levels were studied using Western blot and qPCR. NF-κB activity was studied by luciferase assay. RESULTS High expression of Rab11 was detected in 41.5% (66/159) of tumor specimens. We found a significant correlation between high Rab11 expression and depth of tumor invasion (P=0.004). Rab11 overexpression was observed to promote the growth rate and invasiveness of cancer cells through upregulation of MMP9, cyclin E, and cyclin D1 levels. Rab11 overexpression further elevated NF-κB reporter activity and enhanced p-IκB expression. Use of BAY 11-7082, a noted NF-κB inhibitor, partially abolish...Continue Reading

Citations

Aug 28, 2020·International Journal of Molecular Sciences·Mary J O'Sullivan, Andrew J Lindsay
Feb 21, 2021·Cytokine & Growth Factor Reviews·Chenhe Su, Chunfu Zheng
Mar 31, 2021·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Chenyang QiHuijuan Wu

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Methods Mentioned

BETA
GTPases
transfection
GTPase

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