Rab13 is upregulated during osteoclast differentiation and associates with small vesicles revealing polarized distribution in resorbing cells.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Mirkka J HirvonenH Kalervo Väänänen

Abstract

Osteoclasts are bone-resorbing multinucleated cells that undergo drastic changes in their polarization due to heavy vesicular trafficking during the resorption cycle. These events require the precise orchestration of membrane traffic in order to maintain the unique characteristics of the different membrane domains in osteoclasts. Rab proteins are small GTPases involved in regulation of most, if not all, steps of vesicle trafficking. The investigators studied RAB genes in human osteoclasts and found that at least 26 RABs were expressed in osteoclasts. Out of these, RAB13 gene expression was highly upregulated during differentiation of human peripheral blood monocytic cells into osteoclasts. To study its possible function in osteoclasts, the investigators performed immunolocalization studies for Rab13 and various known markers of osteoclast vesicular trafficking. Rab13 localized to small vesicular structures at the superior parts of the osteoclast between the trans-Golgi network and basolateral membrane domain. Rab13 localization suggests that it is not involved in endocytosis or transcytosis of bone degradation products. In addition, Rab13 did not associate with early endosomes or recycling endosomes labeled with EEA1 or TRITC-c...Continue Reading

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Citations

Jun 19, 2013·FEBS Open Bio·Mirkka J HirvonenKalervo H Väänänen
Sep 24, 2010·PloS One·Karen FullerTim J Chambers
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Mar 7, 2019·Biochemical Society Transactions·Pei Ying NgNathan John Pavlos
Sep 7, 2018·BioMed Research International·Michèle Roy, Sophie Roux
Oct 22, 2020·International Journal of Molecular Sciences·Michèle Roy, Sophie Roux
Dec 24, 2020·European Journal of Cell Biology·Michèle RoySophie Roux

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Methods Mentioned

BETA
GTPases
PCR
electrophoresis

Software Mentioned

Ensembl

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