Rab2 protein enhances coatomer recruitment to pre-Golgi intermediates.

The Journal of Biological Chemistry
E J Tisdale, M R Jackson

Abstract

The Rab2 protein is a resident of pre-Golgi intermediates and required for vesicular transport in the early secretory pathway. We have previously shown that a peptide corresponding to the amino terminus of Rab2 (residues 2-14) arrests protein traffic prior to a rate-limiting event in VSV-G movement through pre-Golgi structures (Tisdale, E. J., and Balch, W. E. (1996) J. Biol. Chem. 271, 29372-29379). To determine the mechanism by which this peptide inhibits transport, we investigated the effect of the Rab2 peptide on the distribution of the beta-COP subunit of coatomer because COPI partially localizes to pre-Golgi intermediates. We found that the peptide caused a dramatic change in the distribution of pre-Golgi intermediates containing beta-COP. A quantitative binding assay was employed to measure recruitment of beta-COP to membrane when incubated with the Rab2 (13-mer). Peptide-treated microsomes showed a 25-70% increase in the level of membrane-associated beta-COP. The enhanced recruitment of coatomer to membrane was specific to the Rab2 (13-mer) and required guanosine 5'-3-O-(thio)triphosphate, ADP ribosylation factor, and protein kinase C-like activity. The ability to enhance beta-COP membrane binding was not limited to the...Continue Reading

References

Jan 1, 1992·Methods in Enzymology·P A RandazzoR A Kahn
Aug 1, 1991·Current Opinion in Cell Biology·H R Pelham
Apr 15, 1991·Biochemical and Biophysical Research Communications·R F BrunsH Nakano
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P Van Der SluijsI Mellman
Nov 15, 1990·Biochemical and Biophysical Research Communications·J M HerbertJ P Maffrand
Dec 1, 1986·The Journal of Cell Biology·V J Allan, T E Kreis
Aug 1, 1995·Current Opinion in Cell Biology·H R Pelham
Jan 20, 1995·The Journal of Biological Chemistry·T S PillayJ M Olefsky
Sep 15, 1994·Annals of the New York Academy of Sciences·H HallerF C Luft
Mar 1, 1994·The Journal of Cell Biology·S Hara-KugeJ E Rothman
Apr 1, 1993·The Journal of Cell Biology·A OprinsJ W Slot
Dec 1, 1995·Cell·J A WhitneyI Mellman
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·E DaroI Mellman
Nov 15, 1996·The Journal of Biological Chemistry·E J Tisdale, W E Balch
Jan 17, 1997·The Journal of Biological Chemistry·M YedovitzkyR Nesher
May 5, 1997·The Journal of Cell Biology·E J TisdaleW E Balch
Sep 4, 1997·Nature·J F PresleyJ Lippincott-Schwartz

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May 6, 2016·Frontiers in Cell and Developmental Biology·Jaakko Saraste
Oct 4, 2013·Small GTPases·Maximiliano Gabriel Gutierrez
Sep 17, 2013·Cell Host & Microbe·Racquel Kim Sherwood, Craig R Roy
Jun 13, 2013·Analytical Biochemistry·Gareth W MorganDavid J Vaux
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Jun 27, 2018·Autophagy·Viktor Karlovich LundOle Kjaerulff
Oct 2, 2019·Frontiers in Cell and Developmental Biology·Peter M Luo, Michael Boyce
Jul 31, 1999·The Journal of Biological Chemistry·P A Zuk, L A Elferink
Jul 23, 2003·The Journal of Biological Chemistry·Ellen J TisdaleCristina R Artalejo
Jul 20, 2012·Physiological Reviews·Elise BalseStéphane N Hatem
Jun 28, 2019·Life Science Alliance·Andrey S SelyuninSomshuvra Mukhopadhyay
Dec 14, 2007·The Biochemical Journal·Hiroyuki SanoGustav E Lienhard
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