RAB2A: a major subacrosomal protein of bovine spermatozoa implicated in acrosomal biogenesis

Biology of Reproduction
Jeremi R MountjoyR Oko

Abstract

The perinuclear theca (PT) of mammalian sperm is a unique subcellular structure encapsulating the nucleus. Compositionally, the PT is made up of at least six prominent polypeptides (60, 36, 31, 28, 24, and 15 kDa), of which only two have been sequence identified, as well as many less prominent ones. As an ongoing process in unveiling the protein composition of the PT, we have uncovered the sequence identity of the prominent 24-kDa polypeptide (PT24). Initial N-terminal sequence analysis obtained by Edman degradation suggested that PT24 is a RAB2 protein. This was corroborated by mass spectrometric analyses of trypsin-digested fragments of PT24, identifying RAB2A of the RAB2 subfamily as the best sequence match. Quadrapole/time-of-flight analysis identified 72%% sequence coverage between PT24 and bull, human, mouse, or rabbit RAB2A. Since a genome search only identified two RAB2 subfamily members, RAB2A and RAB2B, the 72%% sequence coverage of PT24 provides assurance that this protein is RAB2A and not a new RAB2 subfamily member. Furthermore, commercial RAB2A antibodies, raised against oligopeptide fragments in the unique C-terminal region of RAB2A, specifically labeled PT24 on Western blot analysis of PT extracts. These anti-RA...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 1, 1976·Journal of Ultrastructure Research·J L CourtensJ E Fléchon
Jan 1, 1990·Progress in Histochemistry and Cytochemistry·C E SmithJ J Bergeron
Jul 1, 1988·Gamete Research·G E Olson, V P Winfrey
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N TouchotA Tavitian
Mar 1, 1971·The American Journal of Anatomy·F R SusiY Clermont
Apr 1, 1982·The American Journal of Anatomy·X M TangY Clermont
Nov 1, 1983·Journal of Ultrastructure Research·G E OlsonD L Garbers
Apr 1, 1981·The American Journal of Anatomy·M Lalli, Y Clermont
Aug 1, 1995·Experimental Cell Research·M von BülowW W Franke
May 1, 1994·Biology of Reproduction·R Oko, D Maravei
Dec 1, 1995·The Journal of Cell Biology·Y FengA Wandinger-Ness
Dec 15, 1995·Microscopy Research and Technique·R Oko, D Maravei
Aug 26, 1998·Biochimica Et Biophysica Acta·O Martinez, B Goud
Feb 26, 2000·Molecular Biology of the Cell·C BucciB van Deurs
Aug 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·M MichautL S Mayorga
Feb 24, 2001·Methods in Enzymology·Y FengA Wandinger-Ness
Mar 10, 2001·Nature·J B BockR H Scheller
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·M Zerial, H McBride
Mar 27, 2001·The Journal of Cell Biology·A N HumeM C Seabra
Jun 20, 2001·Experimental Cell Research·J Ramalho-SantosG Schatten
Nov 8, 2001·Journal of Molecular Biology·J B Pereira-Leal, M C Seabra
Dec 12, 2001·The Journal of Cell Biology·B ShortF A Barr
Jun 5, 2003·The Journal of Biological Chemistry·P Ronald Tovich, Richard J Oko
Nov 25, 2004·Journal of Cell Science·Bassam R AliMiguel C Seabra
Jul 27, 2005·Biochemical Society Transactions·B R Ali, M C Seabra

❮ Previous
Next ❯

Citations

Apr 13, 2000·International Journal of Andrology·Y ToyamaS Yuasa
Oct 12, 2011·Cell and Tissue Research·Richard OkoAarnoud C van der Spoel
May 17, 2014·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Amanda V AlbuquerqueHélio Chiarini-Garcia
Nov 4, 2009·Journal of Reproductive Immunology·Richard Oko, Peter Sutovsky
Sep 9, 2015·Scientific Reports·Woo-Sung KwonMyung-Geol Pang
Dec 7, 2010·International Journal of Andrology·Y-H LinP-L Kuo
Mar 21, 2016·Journal of Dairy Science·Jing LuKasper Arthur Hettinga
Apr 5, 2014·Genes, Chromosomes & Cancer·Hideki YoshidaHajime Hosoi
May 3, 2013·Systems Biology in Reproductive Medicine·Bahar BehrouziClifford L Librach
Nov 25, 2014·Pathology Oncology Research : POR·Seyedmehdi NourashrafeddinMohammad Nouri
Oct 23, 2015·Scientific Reports·Woo-Sung KwonMyung-Geol Pang
Apr 9, 2017·Molecular Reproduction and Development·Md Saidur RahmanMyung-Geol Pang
Feb 20, 2015·Molecular & Cellular Proteomics : MCP·Woo-Sung KwonMyung-Geol Pang
May 10, 2017·The Journal of Cell Biology·Péter LőrinczGábor Juhász
Feb 11, 2009·Microscopy Research and Technique·Yang YuRichard Oko
Dec 17, 2008·Biology of Reproduction·Dolores D Mruk, Ann S N Lau
Jan 10, 2017·International Journal of Molecular Sciences·Ying-Hung LinChung-Hsin Yeh
Apr 23, 2020·Human Reproduction Update·Christiane PleugerMoira K O'Bryan
Aug 23, 2018·Histochemistry and Cell Biology·Tomohide UnoAkira Mizoguchi
Mar 12, 2021·Journal of Proteome Research·Marcos G CarvalhoFabiana F Souza

❮ Previous
Next ❯

Methods Mentioned

BETA
Electrophoresis
electron microscopy
GTPases
geranylgeranylation

Software Mentioned

Proteinlynx Global Server
MASCOT
Matrix Science
Proteinlynx Global Server2

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.