Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation

Histochemistry and Cell Biology
Hai-Long WangQ Y Sun

Abstract

Rab family members play important roles in membrane trafficking, cell growth, and differentiation. Almost all components of the cell endomembrane system, the nucleus, and the plasma membrane are closely related to RAB proteins. In this study, we investigated the distribution and functions of three members of the Rab family, Rab3A, Rab27A, and Rab35, in mouse oocyte meiotic maturation and activation. The three Rab family members showed different localization patterns in oocytes. Microinjection of siRNA, antibody injection, or inhibitor treatment showed that (1) Rab3A regulates peripheral spindle and cortical granule (CG) migration, polarity establishment, and asymmetric division; (2) Rab27A regulates CG exocytosis following MII-stage oocyte activation; and (3) Rab35 plays an important role in spindle organization and morphology maintenance, and thus meiotic nuclear maturation. These results show that Rab proteins play important roles in mouse oocyte meiotic maturation and activation and that different members exert different distinct functions.

References

Nov 1, 1991·Molecular Reproduction and Development·G T O'NeillM H Kaufman
Dec 24, 1981·Nature·K S CuthbertsonP H Cobbold
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·S M WilsonN A Jenkins
Apr 30, 2002·Developmental Biology·Linda L RunftLisa M Mehlmann
Nov 12, 2002·The EMBO Journal·Toshiaki SakisakaWilliam E Balch
Sep 24, 2005·Nature Cell Biology·Manuel ThéryMichel Bornens
Feb 16, 2006·Reproduction, Fertility, and Development·Allison J Gardner, Janice P Evans
May 11, 2006·Journal of Cell Science·Takashi Tsuboi, Mitsunori Fukuda
Jul 11, 2006·American Journal of Physiology. Cell Physiology·M RupnikR Zorec
Sep 5, 2006·Current Biology : CB·Ilektra KourantiArnaud Echard
Feb 22, 2007·Journal of Cell Science·Mark T W HandleyRobert D Burgoyne
Nov 9, 2007·Journal of Cell Science·Samantha L SchwartzAngela Wandinger-Ness
Jul 9, 2008·The Journal of Cell Biology·Sarah WoolnerWilliam M Bement
Dec 17, 2008·Reproductive Biomedicine Online·Elke HeytensPetra De Sutter
Mar 18, 2009·FEBS Letters·Julien ChevallierJohn F Presley
Jul 16, 2009·Nature Reviews. Molecular Cell Biology·Harald Stenmark
Nov 26, 2009·FEBS Letters·Christelle En Lin ChuaBor Luen Tang
Nov 10, 2010·The Journal of Cell Biology·Mathew P EsteyWilliam S Trimble
Apr 26, 2011·Reproductive Biology and Endocrinology : RB&E·Claudia G PetersenJosé G Franco
Nov 18, 2011·Reproductive Biology and Endocrinology : RB&E·Min Liu
Jan 10, 2012·Current Biology : CB·Laurent ChesneauArnaud Echard
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Matías A BustosClaudia N Tomes
Aug 17, 2012·Cell Cycle·Sen LiQing-Yuan Sun
Jul 16, 2013·Nature Cell Biology·Agathe ChaigneMarie-Emilie Terret
Jul 23, 2013·Nature Cell Biology·Zuzana HolubcováMelina Schuh
Sep 26, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Kexi YiRong Li
May 31, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rujun MaQiang Wang

❮ Previous
Next ❯

Citations

Jul 18, 2016·Experimental Cell Research·Oscar Daniel BelloMarcela A Michaut
Dec 20, 2016·Nature Communications·Liam P CheesemanMelina Schuh
Jul 17, 2018·Biology of Reproduction·Xing Duan, Shao-Chen Sun
Aug 29, 2018·Pest Management Science·Ya-Ting HeQing-Yuan Sun
Jun 16, 2019·Biology of Reproduction·Lisa M MehlmannKatie M Lowther
Jun 18, 2020·Cells·Pika Miklavc, Manfred Frick
May 24, 2021·Experimental Cell Research·Matilde de PaolaMarcela Alejandra Michaut
Jul 7, 2021·Human Reproduction Update·Meng-Meng Shan, Shao-Chen Sun

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.