PMID: 6105876Jul 1, 1980Paper

Rabbit intestinal aminopeptidase N. Purification and molecular properties

Biochimica Et Biophysica Acta
H Feracci, S Maroux

Abstract

The detergent and protease forms of rabbit intestinal aminopeptidase N were purfied for chemical investigations and future specific immunological labeling of the enzyme in situ. The purification of the detergent form required a special technique called 'reverse immunoabsorbant chromatography'. The specific activity of the detergent form finally obtained was identical to that of the protease form. A significant charge micro heterogeneity persisted in the most purified preparations, due probably to a certain level of variability in the sugar moiety. The major proteolytic cleavage which occurred at the hydrophilic-hydrophobic junction of the detergent form during its conversion into the protease form was well defined. But additional splittings probably in C-terminal region of the molecules led to several protease forms differing by their size. The molecular weight assigned to the peptide liberated during the above conversion was overestimated due to preferential detergent binding to hydrophobic structures. The correct value, estimated by a new isotopic dilution method, was 3800 (36-38 residues) for the peptide originating from the rabbit enzyme. The real anchor plunging into the membrane core is possibly still shorter. Comparative...Continue Reading

References

Oct 26, 1977·Biochimica Et Biophysica Acta·E M DanielsenE Dabelsteen
Oct 26, 1976·Biochimica Et Biophysica Acta·C Tanford, J A Reynolds
Nov 1, 1975·FEBS Letters·J M PagèsC Lazdunski
Jun 1, 1972·FEBS Letters·T Ternynck, S Avrameas
Sep 15, 1973·Biochimica Et Biophysica Acta·S MarouxJ Baratti
Jan 1, 1971·Helvetica Chimica Acta·H WackerE A Stein
Mar 1, 1964·Biochemistry·D A YPHANTIS

❮ Previous
Next ❯

Citations

Apr 1, 1988·Journal of Bioenergetics and Biomembranes·S P Shirazi-BeecheyR B Beechey
Jan 1, 1985·The Journal of Membrane Biology·H FeracciS Maroux
Aug 31, 1994·Journal of Biotechnology·C Schmidt-DannertR D Schmid
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·V V EgorovaA M Ugolev
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S P Shirazi-BeecheyR B Beechey
Jan 1, 1994·Biology of the Cell·B O'CallaghanN Morel
Dec 1, 1995·Journal of Gastroenterology·H Terashima, N W Bunnett
Feb 3, 2005·Journal of Neurochemistry·Lucia Chávez-GutiérrezJean-Louis Charli
Jan 31, 1996·Biochimica Et Biophysica Acta·J F PoschetP D Fairclough
Jul 1, 1985·Journal of Pharmaceutical Sciences·R E Stratford, V H Lee
Jan 1, 1995·American Journal of Surgery·I S TaitF C Campbell
Jul 26, 1982·Biochimica Et Biophysica Acta·D GratecosM Sémériva
May 25, 1994·Biochimica Et Biophysica Acta·J I Penny, F C Campbell
Sep 9, 1980·Biochimica Et Biophysica Acta·J P GorvelS Maroux
Sep 1, 1988·Biochimie·S MarouxD Louvard
Aug 3, 2021·Journal of the Endocrine Society·Sasha A S KjeldsenNicolai J Wewer Albrechtsen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.