Rabbit intraocular reactivity to endotoxin measured by slit-lamp biomicroscopy and laser flare photometry

Ophthalmology
Robert B NussenblattMalvina B Eydelman

Abstract

To evaluate the ocular reactivity of the rabbit to an intracameral injection of a dispersive ophthalmic viscosurgical device (OVD) containing various levels of bacterial endotoxin using slit-lamp biomicroscopy and laser flare photometry. Experimental, randomized, masked animal study. Thirty Dutch-Belted rabbits. The rabbits were randomized into 6 groups to receive 0.05 ml of a hydroxypropyl methylcellulose-based dispersive OVD to which had been added one of 5 different doses of bacterial endotoxin ranging from 0.02 to 1.4 endotoxin units (EUs) or a vehicle control to both eyes. The eyes were evaluated for anterior segment inflammation at baseline and 3, 6, 9, 24, 48, and 72 hours after injection using slit-lamp biomicroscopy and laser flare photometry. Corneal clarity and anterior chamber (AC) inflammation. All the corneas remained clear throughout the study. Anterior chamber cells were seen at 6, 9, and 24 hours in 60% to 100% of the eyes intracamerally injected with endotoxin-containing OVD, and the response declined rapidly after 24 hours. A dose-response effect was seen between the concentration of endotoxin and the AC cell response. The aqueous flare response in eyes injected with the 2 highest doses of endotoxin was signi...Continue Reading

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Citations

Jan 9, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Araz SabzevariRassoul Dinarvand
Jul 4, 2012·Ophthalmology·John D Gottsch, Oliver D Schein
May 15, 2012·Ophthalmology·Malvina B EydelmanKesia Y Alexander
Apr 10, 2019·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Vladimir BantseevEvan A Thackaberry
Feb 24, 2018·ILAR Journal·Brian C Gilger
Nov 27, 2016·Japanese Journal of Ophthalmology·Mitsuru Sawa
Mar 21, 2019·Pharmaceutical Development and Technology·Mitra Alami-MilaniMitra Jelvehgari
Aug 20, 2020·Acta Biomaterialia·Lokendrakumar C BenganiJoseph B Ciolino
Dec 22, 2020·ACS Pharmacology & Translational Science·David F WoodwardNeil J Poloso

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