Rabbit Model for in vivo Study of Intervertebral Disc Degeneration and Regeneration.

Journal of Korean Neurosurgical Society
Min Ho KongJeffrey C Wang

Abstract

The purpose of this study is to verify the usefulness of the rabbit model for disc degeneration study. The L1-L2, L2-L3, L3-L4, or L4-L5 lumbar intervertebral disc (IVD) of 9 mature male New Zealand White rabbits were injured by inserting a 16-gauge needle to a depth of 5 mm in the left anterolateral annulus fibrosus while leaving L5-L6 IVD uninjured. Three other rabbits also received intradiscal injections of rabbit disc cells transfected with adenovirus and bone morphogenetic protein-2 (ad-BMP-2) at L4-L5 in addition to injury by 16-gauge needle at the L1-L2 level. Using digitized radiographs, measurements of IVD height were made and analyzed by using the disc height index (DHI). Magnetic resonance imaging (MRI) scans of the injured discs, injected discs, and uninjured L5-L6 discs were performed at 15 weeks post surgery and compared with preoperative MRI scans. All twelve rabbits showed consistent results of disc degeneration within 15 weeks following annular puncture. DHIs of injured discs were significantly lower than that of the uninjured L5-L6 discs (p<0.05). The mean value of disc degeneration grade of injured discs was significantly higher than that of uninjured discs (p<0.05). The injection of disc cell transfected wit...Continue Reading

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Citations

May 29, 2012·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Daniel HaschtmannJivko V Stoyanov
May 23, 2014·The Spine Journal : Official Journal of the North American Spine Society·Zhi-Yuan ShiDi-Ke Ruan
Feb 2, 2013·The Spine Journal : Official Journal of the North American Spine Society·Isaac L MossHoward S An
Jul 27, 2010·The Spine Journal : Official Journal of the North American Spine Society·Huilin YangNabil A Ebraheim
Nov 11, 2011·Journal of Tissue Engineering and Regenerative Medicine·D R PereiraR L Reis
Feb 23, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Bradley J BruggemanBrian D Harfe
Apr 2, 2015·Osteoarthritis and Cartilage·P-P A VergroesenT H Smit
Nov 6, 2014·Journal of Spinal Disorders & Techniques·Robert A HartmanJames D Kang
Apr 25, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Matthew PiazzaLachlan J Smith
Aug 5, 2009·Reproduction in Domestic Animals = Zuchthygiene·E MocéJ S Vicente
Sep 10, 2019·Bio-medical Materials and Engineering·Atsuya WatanabeAntonella Schiavinato
Jul 28, 2019·International Journal of Molecular Sciences·Gauri TendulkarAndreas K Nüssler
Nov 12, 2020·Experimental Biology and Medicine·Demissew Shenegelegn MernClaudius Thomé

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