Rabbit ocular reactivity to bacterial endotoxin contained in aqueous solution and ophthalmic viscosurgical devices

Ophthalmology
Shelley Y BuchenMalvina B Eydelman

Abstract

To describe the ocular reactivity of the rabbit to bacterial endotoxin contained in an aqueous medium and in a cohesive and a dispersive ophthalmic viscosurgical device (OVD). Experimental, randomized animal study. Seventy-five New Zealand white rabbits. This study was performed using 75 rabbits to evaluate the ocular reactivity to bacterial endotoxin contained in Dulbecco's phosphate-buffered saline (DPBS), a cohesive OVD, and a dispersive OVD. For each test material, 25 rabbits were randomized into 5 groups and were exposed to the test material containing 0.75 endotoxin units (EU), 0.25 EU, 0.08 EU, and 0.02 EU of endotoxin or the vehicle control. The rabbits in each group received bilateral intracameral injection of 0.05 ml of the same test material. All eyes were examined by slit-lamp biomicroscopy at baseline, 3, 6, 9, 24, 48, and 72 hours after injection. At 24 and 72 hours, slit-lamp biomicroscopy (and additionally indirect ophthalmoscopy) was performed through dilated pupils. Corneal clouding, anterior chamber (AC) flare, cells and fibrin, vitreous haze and cells, cells and fibrin on lens surface, lens opacities, and onset time. The inflammation seen after exposure to the 3 endotoxin-spiked materials followed the same g...Continue Reading

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Citations

Jul 4, 2012·Ophthalmology·John D Gottsch, Oliver D Schein
May 15, 2012·Ophthalmology·Malvina B EydelmanKesia Y Alexander
Apr 18, 2014·Cutaneous and Ocular Toxicology·David Joseph McCannaMaud B Gorbet
Apr 10, 2019·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Vladimir BantseevEvan A Thackaberry
Mar 4, 2020·Journal of Cataract and Refractive Surgery·Narayan BardoloiHimangshu Das
Aug 20, 2020·Acta Biomaterialia·Lokendrakumar C BenganiJoseph B Ciolino
Mar 17, 2021·Biomaterials·Alexander E G BakerMolly S Shoichet

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