Rabies viruses infect primary cultures of murine, feline, and human microglia and astrocytes

Archives of Virology
N B RayD L Lodmell

Abstract

Recent studies have reported the detection of rabies viral antigens and virions in astrocytes and microglia of rabies-infected animals. As a first step toward understanding whether these glial cells may be involved in rabies virus replication, persistence, and/or pathogenesis, we explored their potential to be infected in vitro. Primary cultures of murine, feline, and human microglia and astrocytes were infected with several different rabies viruses: two unpassaged street virus isolates, a cell culture-adapted strain, and a mouse brain-passaged strain. Infection, as determined by immunofluorescence, was detected in 15 of the 16 (94%) virus-glial cell combinations. Replication of infectious virus, determined by infectivity assay, was detected in 7 of the 8 (88%) virus-cell combinations. These results show that astrocytes and microglia can be infected by rabies viruses, suggesting that they may have a potential role in disease, perhaps contributing to viral spread, persistence and/or neuronal dysfunction.

Citations

Jan 18, 2003·Brain Research. Molecular Brain Research·Matthias TiteuxDenise Paulin
Jun 3, 2014·Retrovirology·John G WalshChristopher Power
Nov 20, 2012·Microbes and Infection·Florence NazéSteven Van Gucht
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Sep 24, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Pingsen ZhaoXianzhu Xia
Oct 19, 2011·Comparative Immunology, Microbiology and Infectious Diseases·Pingsen ZhaoXianzhu Xia
Nov 24, 2020·Journal of Neuroscience Methods·Alexandra Rogers, Kevin T Beier
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Dec 2, 1999·Vaccine·W W LeitnerN P Restifo
Jun 12, 2021·Frontiers in Microbiology·Yang WangXiaofeng Guo

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