Rabphilin involvement in filtration and molecular uptake in Drosophila nephrocytes suggests a similar role in human podocytes.

Disease Models & Mechanisms
Estela Selma-SorianoJosep Redon

Abstract

Drosophila nephrocytes share functional, structural and molecular similarities with human podocytes. It is known that podocytes express the rabphilin 3A (RPH3A)- RAB3A complex, and its expression is altered in mouse and human proteinuric disease. Furthermore, we previously identified a polymorphism that suggested a role for RPH3A protein in the development of urinary albumin excretion. As endocytosis and vesicle trafficking are fundamental pathways for nephrocytes, the objective of this study was to assess the role of the RPH3A orthologue in Drosophila, Rabphilin (Rph), in the structure and function of nephrocytes. We confirmed that Rph is required for the correct function of the endocytic pathway in pericardial Drosophila nephrocytes. Knockdown of Rph reduced the expression of the cubilin and stick and stones genes, which encode proteins that are involved in protein uptake and filtration. We also found that reduced Rph expression resulted in a disappearance of the labyrinthine channel structure and a reduction in the number of endosomes, which ultimately leads to changes in the number and volume of nephrocytes. Finally, we demonstrated that the administration of retinoic acid to IR-Rph nephrocytes rescued some altered aspects,...Continue Reading

References

Mar 7, 1998·The Journal of General Physiology·M E BurnsG J Augustine
Sep 5, 2001·The EMBO Journal·C RaiborgH Stenmark
Feb 11, 2003·Cell Structure and Function·Camilla Raiborg, Harald Stenmark
Aug 26, 2003·The American Journal of Pathology·Maria Pia RastaldiGiuseppe D'Amico
Jan 15, 2004·The Journal of Biological Chemistry·Mitsunori FukudaAkitsugu Yamamoto
Jun 11, 2008·Nature Reviews. Genetics·Karen Niederreither, Pascal Dollé
Sep 29, 2009·Current Opinion in Genetics & Development·Barry Denholm, Helen Skaer
Feb 18, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Thaneas PrabakaranPierre J Verroust
Apr 12, 2012·The Journal of Biological Chemistry·Sandeep K MallipattuJohn C He
Dec 25, 2012·Journal of the American Society of Nephrology : JASN·Fujian ZhangZhe Han
Jan 8, 2013·Journal of the American Society of Nephrology : JASN·Fujian ZhangZhe Han
Jan 8, 2013·Journal of the American Society of Nephrology : JASN·Jianchun ChenJian-Kang Chen
Jan 22, 2013·Journal of the American Society of Nephrology : JASN·Jianbo Na, Ross Cagan
Mar 16, 2013·Journal of the American Society of Nephrology : JASN·Wibke BechtelTobias B Huber
Jun 12, 2014·PloS One·Vannina G MarrachelliJosep Redon
Apr 9, 2015·Frontiers in Medicine·Sandeep K Mallipattu, John Cijiang He
Jun 12, 2016·Journal of the American Society of Nephrology : JASN·Sandeep K MallipattuJohn C He
Nov 18, 2016·American Journal of Physiology. Renal Physiology·Sandeep K MallipattuJohn C He
Nov 20, 2016·Journal of the American Society of Nephrology : JASN·Yulong FuZhe Han
Dec 10, 2016·Journal of the American Society of Nephrology : JASN·Tobias HermleFriedhelm Hildebrandt
Feb 10, 2017·Cell and Tissue Research·Yulong FuZhe Han
Apr 13, 2017·Cell and Tissue Research·Martin Helmstädter, Matias Simons
Jul 19, 2017·Cellular and Molecular Life Sciences : CMLS·Florian HochapfelMichael P Krahn
Oct 24, 2017·Scientific Reports·Lisa GianeselloDorella Del Prete
Dec 23, 2017·Frontiers in Pediatrics·Martin HelmstädterTobias Hermle
Apr 17, 2018·Journal of Visualized Experiments : JoVE·Estela Selma-SorianoBeatriz Llamusi
Aug 26, 2018·Journal of the American Society of Nephrology : JASN·Yiqing GuoSandeep K Mallipattu

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Citations

Jul 25, 2021·International Journal of Molecular Sciences·Olga Martinez-ArroyoJosep Redon
Jul 29, 2021·Scientific Reports·Estela Selma-SorianoJosep Redón
Jul 29, 2021·The New England Journal of Medicine·Aurelie EdwardsThomas Benzing

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Datasets Mentioned

BETA
2722114

Methods Mentioned

BETA
GTPase
Fluorescence
confocal microscopy

Software Mentioned

ZEN
Imaris

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