Rac1-null mouse embryonic fibroblasts are motile and respond to platelet-derived growth factor

Molecular Biology of the Cell
Luis VidaliDavid J Kwiatkowski

Abstract

Previous studies of Rac1 in fibroblasts have used dominant negative constructs, which may have nonspecific effects. We used a conditional Rac1 allele to critically examine Rac1 function in mouse fibroblasts. Lack of Rac1 had dramatic effects on nonconfluent cells, which were elongated and had extensive blebbing, but no lamellipodia or ruffle formation. However, Rac1-null fibroblasts translocated using pseudopodia-like protrusions without lamellipodia, migrating toward a platelet-derived growth factor (PDGF) gradient as efficiently as their wild-type counterparts. Rac1-null fibroblasts closed wounds in vitro and spread on a fibronectin substrate, although at a slower rate than wild-type cells. However, Rac1-null cells were markedly impaired in proliferation, with a defect in G1 to S transition, although they were capable of surviving in culture for more than 2 wk. These results refine our understanding of the functions of Rac1, indicate that lamellipodia formation is not required for cell motility, and show that PDGF-induced chemotaxis can occur in the absence of both lamellipodia and Rac1.

References

Jan 31, 1992·Biochemical and Biophysical Research Communications·K HiraokaJ Didsbury
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F DolbeareJ W Gray
Mar 30, 1995·Nature·R G QiuM Symons
Aug 15, 1997·The Journal of Biological Chemistry·L HaatajaN Heisterkamp
Apr 18, 1998·The EMBO Journal·T AzumaD J Kwiatkowski
Jun 12, 1998·The Journal of Cell Biology·W E AllenG E Jones
Jul 11, 1998·Molecular Biology of the Cell·L S PriceG M Bokoch
Jul 29, 1998·The Journal of Cell Biology·E A ClarkR O Hynes
Mar 24, 1999·The Journal of Cell Biology·C D Nobes, A Hall
Feb 23, 2002·Trends in Cell Biology·J Victor SmallKlemens Rottner
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael PeitzFrank Edenhofer
Jul 9, 2002·Genes & Development·Anja Schmidt, Alan Hall
Aug 16, 2002·Nature·Sharon EdenMarc W Kirschner
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Jan 28, 2003·The Journal of Biological Chemistry·Xiangrong GuoMingyao Liu
Feb 5, 2003·The Journal of Cell Biology·Tatyana M SvitkinaGary G Borisy
Mar 15, 2003·Science·Aaron F StraightTimothy J Mitchison
Mar 19, 2003·The Journal of Cell Biology·Danijela VignjevicGary G Borisy
May 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael GlogauerDavid J Kwiatkowski
Oct 17, 2003·The Journal of Clinical Investigation·Hongbing ZhangDavid J Kwiatkowski
Oct 18, 2003·Science·Marita J WalmsleyVictor L J Tybulewicz
Feb 7, 2004·The EMBO Journal·Anika SteffenTheresia E B Stradal
Mar 30, 2004·Nature Cell Biology·Metello InnocentiGiorgio Scita
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Pascal VallottonGaudenz Danuser
Nov 19, 2004·Journal of Muscle Research and Cell Motility·John LimouzeJames R Sellers

❮ Previous
Next ❯

Citations

Jul 26, 2011·Histochemistry and Cell Biology·Sara SolinetMaría Leiza Vitale
Apr 26, 2007·Bulletin of Mathematical Biology·Alexandra JilkineLeah Edelstein-Keshet
Jun 3, 2008·Nature Cell Biology·Petra BeliMetello Innocenti
Oct 12, 2010·Nature Cell Biology·Sonia Castillo-LluvaAngeliki Malliri
Jul 16, 2009·Nature Reviews. Molecular Cell Biology·Ryan J PetrieKenneth M Yamada
Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tanja MaritzenVolker Haucke
Aug 18, 2006·Molecular Biology of the Cell·Christine E PullarR Rivkah Isseroff
Aug 12, 2009·Plant Physiology·Luis VidaliMagdalena Bezanilla
Feb 22, 2012·Biochemistry. Biokhimii︠a︡·A V Vorotnikov
Jan 30, 2010·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Linda SchneiderSøren T Christensen
Jan 8, 2009·BMC Developmental Biology·Thomas U GreinerHenrik Semb
May 29, 2008·Journal of Cell Science·Julia MellerMartin Alexander Schwartz
Oct 23, 2008·Journal of Cell Science·Stephen D SmithAnne J Ridley
May 19, 2010·PloS One·Patrycja M DubieleckaLeszek Kotula
Sep 25, 2010·Journal of Visualized Experiments : JoVE·Steve P HawleyNina Jones
Apr 9, 2008·Cellular and Molecular Life Sciences : CMLS·C DowneyM D Basson
Jan 31, 2013·The Journal of Biological Chemistry·Fumiyuki SanematsuYoshinori Fukui
Apr 30, 2008·FEBS Letters·Markus Ladwein, Klemens Rottner
Jun 11, 2008·The Journal of Cell Biology·Oliver T Fackler, Robert Grosse
Apr 8, 2009·The American Journal of Pathology·Shangxi LiuAndrew Leask
May 13, 2009·The International Journal of Biochemistry & Cell Biology·Jan FaixKlemens Rottner
Oct 10, 2007·Journal of Structural Biology·Roman I KoningA Mieke Mommaas
Dec 13, 2006·Trends in Cell Biology·Lei Wang, Yi Zheng
Sep 13, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Xunwei WuCord Brakebusch
Feb 27, 2007·Journal of Thrombosis and Haemostasis : JTH·S D J CalaminusL M Machesky
Jan 25, 2012·European Journal of Oral Sciences·Zhan HuangMalcolm L Snead
Jul 14, 2012·Small GTPases·Ang Li, Laura M Machesky
Apr 9, 2011·Molecular Cell·Abdelhafid SaciChristopher L Carpenter
Dec 17, 2014·Biochemical and Biophysical Research Communications·Radwan Abu-Issa
Oct 3, 2009·Experimental Cell Research·Magnus C LydolphAtsuko Yoneda
Jun 27, 2009·Biochemical and Biophysical Research Communications·Verena NiggliSarah Affentranger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.