Rac3-induced neuritogenesis requires binding to Neurabin I

Molecular Biology of the Cell
Donata OrioliFiorenzo A Peverali

Abstract

Rac3, a neuronal GTP-binding protein of the Rho family, induces neuritogenesis in primary neurons. Using yeast two-hybrid analysis, we show that Neurabin I, the neuronal F-actin binding protein, is a direct Rac3-interacting molecule. Biochemical and light microscopy studies indicate that Neurabin I copartitions and colocalizes with Rac3 at the growth cones of neurites, inducing Neurabin I association to the cytoskeleton. Moreover, Neurabin I antisense oligonucleotides abolish Rac3-induced neuritogenesis, which in turn is rescued by exogenous Neurabin I but not by Neurabin I mutant lacking the Rac3-binding domain. These results show that Neurabin I mediates Rac3-induced neuritogenesis, possibly by anchoring Rac3 to growth cone F-actin.

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Citations

Aug 19, 2007·Molecular Biology of the Cell·Frédéric CauseretMargareta Nikolic
Feb 1, 2011·Brain : a Journal of Neurology·Karsten RuscherTadeusz Wieloch
Aug 8, 2007·Journal of Neuroscience Research·Masashi Kishi
Sep 29, 2018·PLoS Genetics·Steffen NørgaardRoger Pocock
Apr 8, 2011·Journal of Biomolecular Structure & Dynamics·Shagufta H KhanRaj Kumar

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