Racemization in cataractous lens from diabetic and aging individuals: analysis of Asp 58 residue in αA-crystallin.

Aging
Xiang-Jia ZhuYi Lu

Abstract

Cataract is the leading cause of visual impairment globally. Racemization of lens proteins may contribute to cataract formation in aging individuals. As a special type of age-related cataract (ARC), diabetic cataract (DC) is characterized by the early onset of cortical opacification and finally developed into a mixed type of cortical and nuclear opacification. We compared racemization of Asp 58 residue, a hotspot position in αA-crystallin, from the cortex and nucleus of diabetic and age-matched senile cataractous lenses, by identifying L-Asp/L-isoAsp/D-Asp/D-isoAsp by mass spectrometry. Compared to nondiabetic cataractous lenses, DC lenses showed a significantly increased cortex/nucleus ratio of D-Asp 58, which originated primarily from an increased percentage of D-Asp 58 in the lens cortex of DC. Moreover, patients diagnosed with diabetes for over 10 years showed a lower cortex/nucleus ratio of D-isoAsp 58 in the lens compared with those who had a shorter duration of diabetes, which originated mainly from an increased percentage of D-isoAsp 58 in the lens nucleus of DC with increasing time of hyperglycemia. Further analysis confirmed decreased protein solubility in diabetic cataractous lenses. The different racemization patter...Continue Reading

References

Feb 5, 1999·Progress in Retinal and Eye Research·T J Jacob
Dec 22, 1999·Biochemical and Biophysical Research Communications·N FujiiM Akaboshi
Feb 28, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eszter Herczenik, Martijn F B G Gebbink
Jun 24, 2008·Protein Science : a Publication of the Protein Society·Takumi TakataKirsten J Lampi
May 26, 2009·Biochimica Et Biophysica Acta·K Krishna Sharma, Puttur Santhoshkumar
May 18, 2010·Diabetes/metabolism Research and Reviews·Irina G ObrosovaPeter F Kador
Jul 17, 2010·Journal of Ophthalmology·Andreas Pollreisz, Ursula Schmidt-Erfurth
Oct 30, 2010·Circulation Research·Ferdinando Giacco, Michael Brownlee
Mar 16, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R Michael, A J Bron
Nov 13, 2012·Experimental Eye Research·Michelle Y S HooiRoger J W Truscott
Jan 25, 2014·Proteomics. Clinical Applications·Roger J W Truscott, Michael G Friedrich
Jun 1, 2010·Journal of Diabetes Investigation·Eiichi Araki, Takeshi Nishikawa
Apr 8, 2015·Mechanisms of Ageing and Development·Valerie VanhoorenBertrand Friguet
Aug 16, 2015·Biochimica Et Biophysica Acta·Noriko FujiiKenzo Aki
Jun 28, 2017·JAMA : the Journal of the American Medical Association·Limin WangLinhong Wang
Oct 11, 2017·Journal of Cellular and Molecular Medicine·Xiang-Jia ZhuYi Lu
Nov 18, 2017·Seminars in Ophthalmology·Scott R PetersonJennifer K Sun
Aug 27, 2018·Experimental Eye Research·Eloy Bejarano, Allen Taylor

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