Racemization of undesired enantiomers: Immobilization of mandelate racemase and application in a fixed bed reactor

Biotechnology Journal
Katarzyna WrzosekAndreas Seidel-Morgenstern

Abstract

Production of optically pure products can be based on simple unselective synthesis of racemic mixtures combined with a subsequent separation of the enantiomers; however, this approach suffers from a 50% yield limitation which can be overcome by racemization of the undesired enantiomer and recycling. Application of biocatalyst for the racemization steps offers an attractive option for high-yield manufacturing of commercially valuable compounds. Our work focuses on exploiting the potential of racemization with immobilized mandelate racemase. Immobilization of crude mandelate racemase via covalent attachment was optimized for two supports: Eupergit(®) CM and CNBr-activated Sepharose 4 Fast Flow. To allow coupling of enzymatic reaction with enantioselective chromatography, a mobile phase composition compatible with both processes was used in enzymatic reactor. Kinetic parameters obtained analyzing experiments carried out in a batch reactor could be successfully used to predict fixed-bed reactor performance. The applicability of the immobilized enzyme and the determined kinetic parameters were validated in transient experiments recording responses to pulse injections of R-mandelic acid. The approach investigated can be used for futh...Continue Reading

References

Jul 16, 1985·Biochemistry·C P WhitmanG L Kenyon
Aug 13, 1999·Journal of Chromatography. a·J B Wheatley, D E Schmidt
Mar 20, 2002·Biochemistry·Martin St Maurice, Stephen L Bearne
Aug 14, 2003·Chemical Reviews·Oscar Pàmies, Jan-E Bäckvall
Oct 2, 2004·Biotechnology Progress·Aránzazu Gómez de SeguraFrancisco J Plou
Nov 23, 2007·Biochemical Society Transactions·C MateoJ M Guisán
Jul 11, 2009·Protein Expression and Purification·Ariun Narmandakh, Stephen L Bearne
Aug 13, 2013·Methods in Molecular Biology·Jose M Guisan
Jan 17, 2014·Journal of Chemical Biology·Ahmad Abolpour HomaeiRoberto Stevanato
Mar 3, 2015·Journal of the American Chemical Society·Oscar Verho, Jan-E Bäckvall

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Citations

Dec 12, 2020·Engineering in Life Sciences·Thiane CarneiroAndreas Seidel-Morgenstern

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